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JRK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

JRK Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all JRK antibodies →
$725.00each
Catalog Number: LS-G120031-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human JRK (NM_001077527), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Jerky (mouse) homolog | Jerky protein homolog | JH8 | Jerky homolog (mouse) | JRK
Origin Species
Human
NCBI GenBank
NM_003724
SwissProt
O75564

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASKPAAGKS RGEKRKRVVL TLKEKIDICT RLEKGESRKA LMQEYNVGMS TLYDIRAHKA
61QLLRFFASSD SNKALEQRRT LHTPKLEHLD RVLYEWFLGK RSEGVPVSGP MLIEKAKDFY
121EQMQLTEPCV FSGGWLWRFK ARHGIKKLDA SSEKQSADHQ AAEQFCAFFR SLAAEHGLSA
181EQVYNADETG LFWRCLPNPT PEGGAVPGPK QGKDRLTVLM CANATGSHRL KPLAIGKCSG
241PRAFKGIQHL PVAYKAQGNA WVDKEIFSDW FHHIFVPSVR EHFRTIGLPE DSKAVLLLDS
301SRAHPQEAEL VSSNVFTIFL PASVASLVQP MEQGIRRDFM RNFINPPVPL QGPHARYNMN
361DAIFSVACAW NAVPSHVFRR AWRKLWPSVA FAEGSSSEEE LEAECFPVKP HNKSFAHILE
421LVKEGSSCPG QLRQRQAASW GVAGREAEGG RPPAATSPAE VVWSSEKTPK ADQDGGGDPG
481EGEEVAWEQA AVAFDAVLRF AERQPCFSAQ EVGQLRALRA VFRSQQQETV GLEDVVVTSP
541EELAIPKCCL EASTET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

556 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.