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JMY Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

JMY Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G129149-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human JMY (NM_152405), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
JMY | WHDC1L3
Origin Species
Human
SwissProt
Q8N9B5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFALEETLE SDWVAVRPHV FDEREKHKFV FIVAWNEIEG KFAITCHNRT AQRQRSGSRE
61QAGARGGAEA GGAASDGSRG PGSPAGRGRP EATASATLVR SPGPRRSSAW AEGGSPRSTR
121SLLGDPRLRS PGSKGAESRL RSPVRAKPIP GQKTSEADDA AGAAAAAARP APREAQVSSV
181RIVSASGTVS EEIEVLEMVK EDEAPLALSD AEQPPPATEL ESPAEECSWA GLFSFQDLRA
241VHQQLCSVNS QLEPCLPVFP EEPSGMWTVL FGGAPEMTEQ EIDTLCYQLQ VYLGHGLDTC
301GWKILSQVLF TETDDPEEYY ESLSELRQKG YEEVLQRARK RIQELLDKHK NTESMVELLD
361LYQLEDEAYS SLAEATTELY QYLLQPFRDM RELAMLRRQQ IKISMENDYL GPRRIESLQK
421EDADWQRKAH MAVLSIQDLT VKYFEITAKA QKAVYDRMRA DQKKFGKASW AAAAERMEKL
481QYAVSKETLQ MMRAKEICLE QRKHALKEEM QSLRGGTEAI ARLDQLEADY YDLQLQLYEV
541QFEILKCEEL LLTAQLESIK RLISEKRDEV VYYDTYESME AMLEKEEMAA SAYLQREELQ
601KLQQKARQLE ARRGRVSAKK SYLRNKKEIC IAKHNEKIQQ RTRIEDEYRT HHTVQLKREK
661LHDEEERKSA WVSQERQRTL DRLRTFKQRY PGQVILKSTR LRLAHARRKG AASPVLQEDH
721CDSLPSVLQV EEKTEEVGEG RVKRGPSQTT EPQSLVQLED TSLTQLEATS LPLSGVTSEL
781PPTISLPLLN NNLEPCSVTI NPLPSPLPPT PPPPPPPPPP PPPPPLPVAK DSGPETLEKD
841LPRKEGNEKR IPKSASAPSA HLFDSSQLVS ARKKLRKTAE GLQRRRVSSP MDEVLASLKR
901GSFHLKKVEQ RTLPPFPDED DSNNILAQIR KGVKLKKVQK DVLRESFTLL PDTDPLTRSI
961HEALRRIKEA SPESEDEEEA LPCTDWEN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

988 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.