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JMJD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

JMJD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all JMJD7 antibodies →
$725.00each
Catalog Number: LS-G116192-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human JMJD7 (NM_005090), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CPLA2-BETA | JMJD7 | Jumonji domain containing 7
Origin Species
Human
NCBI GenBank
NM_005090
SwissProt
P0C870

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEAALEAVR SELREFPAAA RELCVPLAVP YLDKPPTPLH FYRDWVCPNR PCIIRNALQH
61WPALQKWSLP YFRATVGSTE VSVAVTPDGY ADAVRGDRFM MPAERRLPLS FVLDVLEGRA
121QHPGVLYVQK QCSNLPSELP QLLPDLESHV PWASEALGKM PDAVNFWLGE AAAVTSLHKD
181HYENLYCVVS GEKHFLFHPP SDRPFIPYEL YTPATYQLTE EGTFKVVDEE AMEKAEVSRT
241CLLTVRVLQA HRLPSKDLVT PSDCYVTLWL PTACSHRLQT RTVKNSSSPV WNQSFHFRIH
301RQLKNVMELK VFDQDLVTGD DPVLSVLFDA GTLRAGEFRR ESFSLSPQGE GRLEVEFRLQ
361SLADRGEWLV SNGVLVAREL SCLHVQLEET GDQKSSEHRV QLVVPGSCEG PQEASVGTGT
421FCFHCPACWE QELSIRLQDA PEEQLKAPLS ALPSGQVVRL VFPTSQEPLM RVELKKEAGL
481RELAVRLGFG PCAEEQAFLS RRKQVVAAAL RQALQLDGDL QEDEIPVVAI MATGGGIRAM
541TSLYGQLAGL KELGLLDCVS YITGASGSTW ALANLYEDPE WSQKDLAGPT ELLKTQVTKN
601KLGVLAPSQL QRYRQELAER ARLGYPSCFT NLWALINEAL LHDEPHDHKL SDQREALSHG
661QNPLPIYCAL NTKGQSLTTF EFGEWCEFSP YEVGFPKYGA FIPSELFGSE FFMGQLMKRL
721PESRICFLEG IWSNLYAANL QDSLYWASEP SQFWDRWVRN QANLDKEQVP LLKIEEPPST
781AGRIAEFFTD LLTWRPLAQA THNFLRGLHF HKDYFQHPHF STWKATTLDG LPNQLTPSEP
841HLCLLDVGYL INTSCLPLLQ PTRDVDLILS LDYNLHGAFQ QLQLLGRFCQ EQGIPFPPIS
901PSPEEQLQPR ECHTFSDPTC PGAPAVLHFP LVSDSFREYS APGVRRTPEE AAAGEVNLSS
961SDSPYHYTKV TYSQEDVDKL LHLTHYNVCN NQEQLLEALR QAVQRRRQRR PH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1012 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.