Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
JEAP / AMOTL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107606-100
Catalog Number: LS-G107606
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Angiomotin like 1(AMOTL1) (NM_130847), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AMOTL1 | Angiomotin like 1 | Angiomotin-like protein 1 | JEAP
- Origin Species
- Human
- SwissProt
- Q8IY63
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 106.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWRAKLRRGT CEPAVKGSPS ACYSPSSPVQ VLEDSTYFSP DFQLYSGRHE TSALTVEATS61SIREKVVEDP LCNFHSPNFL RISEVEMRGS EDAAAGTVLQ RLIQEQLRYG TPTENMNLLA121IQHQATGSAG PAHPTNNFSS TENLTQEDPQ MVYQSARQEP QGQEHQVDNT VMEKQVRSTQ181PQQNNEELPT YEEAKAQSQF FRGQQQQQQQ QGAVGHGYYM AGGTSQKSRT EGRPTVNRAN241SGQAHKDEAL KELKQGHVRS LSERIMQLSL ERNGAKQHLP GSGNGKGFKV GGGPSPAQPA301GKVLDPRGPP PEYPFKTKQM MSPVSKTQEH GLFYGDQHPG MLHEMVKPYP APQPVRTDVA361VLRYQPPPEY GVTSRPCQLP FPSTMQQHSP MSSQTSSASG PLHSVSLPLP LPMALGAPQP421PPAASPSQQL GPDAFAIVER AQQMVEILTE ENRVLHQELQ GYYDNADKLH KFEKELQRIS481EAYESLVKST TKRESLDKAM RNKLEGEIRR LHDFNRDLRD RLETANRQLS SREYEGHEDK541AAEGHYASQN KEFLKEKEKL EMELAAVRTA SEDHRRHIEI LDQALSNAQA RVIKLEEELR601EKQAYVEKVE KLQQALTQLQ SACEKREQME RRLRTWLERE LDALRTQQKH GNGQPANMPE661YNAPALLELV REKEERILAL EADMTKWEQK YLEESTIRHF AMNAAATAAA ERDTTIINHS721RNGSYGESSL EAHIWQEEEE VVQANRRCQD MEYTIKNLHA KIIEKDAMIK VLQQRSRKDA781GKTDSSSLRP ARSVPSIAAA TGTHSRQTSL TSSQLAEEKK EEKTWKGSIG LLLGKEHHEH841ASAPLLPPPP TSALSSIAST TAASSAHAKT GSKDSSTQTD KSAELFWPSM ASLPSRGRLS901TTPAHSPVLK HPAAKGTAEK LENSPGHGKS PDHRGRVSSL LHKPEFPDGE MMEVLITRTRPLEQKLISEEDLAANDILDYKDDDDKV
956 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.