LSBio
JAKMIP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

JAKMIP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G124129-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human JAKMIP3 (NM_001105521), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C10orf14 | C10orf39 | BA140A10.5 | JAKMIP3 | NECC2 | Jamip3 | KIAA4091
Origin Species
Human
NCBI GenBank
NM_001105521
SwissProt
Q5VZ66

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKRGMSSRA KGDKAEALAA LQAANEDLRA KLTDIQIELQ QEKSKVSKVE REKNQELRQV
61REHEQHKTAV LLTELKTKLH EEKMKELQAV RETLLRQHEA ELLRVIKIKD NENQRLQALL
121SALRDGGPEK VKTVLLSEAK EEAKKGFEVE KVKMQQEISE LKGAKRQVEE ALTLVIQADK
181IKAAEIRSVY HLHQEEITRI KKECEREIRR LMEEIKFKDR AVFVLERELG VQAGHAQRLQ
241LQKEALDEQL SQVREADRHP GSPRRELPHA AGAGDASDHS GSPEQQLDEK DARRFQLKIA
301ELSAIIRKLE DRNALLSEER NELLKRVREA ESQYKPLLDK NKRLSRKNED LSHALRRMEN
361KLKFVTQENI EMRQRAGIIR RPSSLNDLDQ SQDEREVDFL KLQIVEQQNL IDELSKTLET
421AGYVKSVLER DKLLRFRKQR KKMAKLPKPV VVETFFGYDE EASLESDGSS VSYQTDRTDQ
481TPCTPDDDLE EGMAKEETEL RFRQLTMEYQ ALQRAYALLQ EQVGGTLDAE REVKTREQLQ
541AEVQRAQARI EDLEKALAEQ GQDMKWIEEK QALYRRNQEL VEKIKQMETE EARLRHEVQD
601ARDQNELLEF RILELEERER KSPAISFHHT PFVDGKSPLQ VYCEAEGVTD IVVAELMKKL
661DILGDNANLT NEEQVVVIQA RTVLTLAEKW LQQIEETEAA LQRKMVDLES EKELFSKQKG
721YLDEELDYRK QALDQANKHI LELEAMLYDA LQQEAGAKVA ELLSEEEREK LKVAVEQWKR
781QVMSELRERD AQILRERMEL LQLAQQRIKE LEERIEAQKR QIKELEEKFL FLFLFFSLAF
841ILWS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

844 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.