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ITPRIPL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ITPRIPL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ITPRIPL2 antibodies →
$504.00each
Catalog Number: LS-G120520-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITPRIPL2 (NM_001034841), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ITPRIPL2
Origin Species
Human
NCBI GenBank
NM_001034841

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVHYTLNLR VFWPLVTGLC TALVCLYHVL RGSGGARAEP ADGVDGGFPL LKVAVLLLLS
61YVLLRCRHAV RQRFLPGSPR LEGHAAFSSR HFREPGLSIL LESYYEHEVR LSPHVLGHSK
121AHVSRIVGEL VRAGRARGSP GLIPGGALAL AFRGDFIQVG SAYEQHKIRR PDSFDVLVPL
181RLPPLVALEP RSLGEEPALA PAFRGCFLCA LKAPPSPSGA SGGHWLRDCK PFADAFCVDV
241RGRRHLSATL VLRWFQSHLQ RSLATVRYSL EGRCRVTLTP GGLEQPPTLH ILPCRTDYGC
301CRLSMAVRLI PAVHLGDGVF LVAPPPPPLP SAPLLELPEG LRAEALWGVN TARQEQKLLS
361WLQERAAPGA CYLKCLQLLK ALRDLGARGL DSAAATQWGR ILSSYVLKTV LLAVLLRKGA
421PGQGWDEEHL GRCLEELVQF LRDCLLRRHT LFHCVLGPGG AAAEVGPLPK ALREAAPVDL
481LAAFDGHARE LAAARLLSTW QRLPQLLRAY GGPRYLARCP PPRSQRTQGF LEGEP
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

535 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.