LSBio
ITPRIP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ITPRIP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ITPRIP antibodies →
$504.00each
Catalog Number: LS-G102131-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITPRIP (NM_033397), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA127L20 | ITPRIP | Protein DANGER | RP11-127L20.4 | BA127L20.2 | DANGER | KIAA1754
Origin Species
Human
SwissProt
Q8IWB1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAMGLFRVCL VVVTAIINHP LLFPRENATV PENEEEIIRK MQAHQEKLQL EQLRLEEEVA
61RLAAEKEALE QVAEEGRQQN ETRVAWDLWS TLCMILFLMI EVWRQDHQEG PSPECLGGEE
121DELPGLGGAP LQGLTLPNKA TLGHFYERCI RGATADAART REFLEGFVDD LLEALRSLCN
181RDTDMEVEDF IGVDSMYENW QVDRPLLCHL FVPFTPPEPY RFHPELWCSG RSVPLDRQGY
241GQIKVVRADG DTLSCICGKT KLGEDMLCLL HGRNSMAPPC GDMENLLCAT DSLYLDTMQV
301MKWFQTALTR AWKGIAHKYE FDLAFGQLDS PGSLKIKFRS GKFMPFNLIP VIQCDDSDLY
361FVSHLPREPS EGTPASSTDW LLSFAVYERH FLRTTLKALP EGACHLSCLQ IASFLLSKQS
421RLTGPSGLSS YHLKTALLHL LLLRQAADWK AGQLDARLHE LLCFLEKSLL QKKLHHFFIG
481NRKVPEAMGL PEAVLRAEPL NLFRPFVLQR SLYRKTLDSF YEMLKNAPAL ISEYSLHVPS
541DQPTPKS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

547 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.