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ITPKC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ITPKC Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109536-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IP3KC(ITPKC) (NM_025194), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
InsP 3 kinase C | IP3-3KC | InsP 3-kinase C | IP3KC | IP3K C | IP3 3-kinase C | ITPKC
Origin Species
Human
NCBI GenBank
AJ290975
SwissProt
Q96DU7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRCPCRGSL NEAEAGALPA AARMGLEAPR GGRRRQPGQQ RPGPGAGAPA GRPEGGGPWA
61RTEGSSLHSE PERAGLGPAP GTESPQAEFW TDGQTEPAAA GLGVETERPK QKTEPDRSSL
121RTHLEWSWSE LETTCLWTET GTDGLWTDPH RSDLQFQPEE ASPWTQPGVH GPWTELETHG
181SQTQPERVKS WADNLWTHQN SSSLQTHPEG ACPSKEPSAD GSWKELYTDG SRTQQDIEGP
241WTEPYTDGSQ KKQDTEAARK QPGTGGFQIQ QDTDGSWTQP STDGSQTAPG TDCLLGEPED
301GPLEEPEPGE LLTHLYSHLK CSPLCPVPRL IITPETPEPE AQPVGPPSRV EGGSGGFSSA
361SSFDESEDDV VAGGGGASDP EDRSGSKPWK KLKTVLKYSP FVVSFRKHYP WVQLSGHAGN
421FQAGEDGRIL KRFCQCEQRS LEQLMKDPLR PFVPAYYGMV LQDGQTFNQM EDLLADFEGP
481SIMDCKMGSR TYLEEELVKA RERPRPRKDM YEKMVAVDPG APTPEEHAQG AVTKPRYMQW
541RETMSSTSTL GFRIEGIKKA DGTCNTNFKK TQALEQVTKV LEDFVDGDHV ILQKYVACLE
601ELREALEISP FFKTHEVVGS SLLFVHDHTG LAKVWMIDFG KTVALPDHQT LSHRLPWAEG
661NREDGYLWGL DNMICLLQGL AQS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

683 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.