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ITPKB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ITPKB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ITPKB antibodies →
$725.00each
Catalog Number: LS-G118114-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITPKB (NM_002221), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IP3 3-kinase B | IP3K-B | ITPKB | IP3-3KB | IP3KB | InsP 3-kinase B | IP3K | IP3K B | PIG37
Origin Species
Human
NCBI GenBank
NM_002221
SwissProt
P27987

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVYCYALNS LVIMNSANEM KSGGGPGPSG SETPPPPRRA VLSPGSVFSP GRGASFLFPP
61AESLSPEEPR SPGGWRSGRR RLNSSSGSGS GSSGSSVSSP SWAGRLRGDR QQVVAAGTLS
121PPGPEEAKRK LRILQRELQN VQVNQKVGMF EAHIQAQSSA IQAPRSPRLG RARSPSPCPF
181RSSSQPPGRV LVQGARSEER RTKSWGEQCP ETSGTDSGRK GGPSLCSSQV KKGMPPLPGR
241AAPTGSEAQG PSAFVRMEKG IPASPRCGSP TAMEIDKRGS PTPGTRSCLA PSLGLFGASL
301TMATEVAARV TSTGPHRPQD LALTEPSGRA RELEDLQPPE ALVERQGQFL GSETSPAPER
361GGPRDGEPPG KMGKGYLPCG MPGSGEPEVG KRPEETTVSV QSAESSDSLS WSRLPRALAS
421VGPEEARSGA PVGGGRWQLS DRVEGGSPTL GLLGGSPSAQ PGTGNVEAGI PSGRMLEPLP
481CWDAAKDLKE PQCPPGDRVG VQPGNSRVWQ GTMEKAGLAW TRGTGVQSEG TWESQRQDSD
541ALPSPELLPQ DQDKPFLRKA CSPSNIPAVI ITDMGTQEDG ALEETQGSPR GNLPLRKLSS
601SSASSTGFSS SYEDSEEDIS SDPERTLDPN SAFLHTLDQQ KPRVSKSWRK IKNMVHWSPF
661VMSFKKKYPW IQLAGHAGSF KAAANGRILK KHCESEQRCL DRLMVDVLRP FVPAYHGDVV
721KDGERYNQMD DLLADFDSPC VMDCKMGIRT YLEEELTKAR KKPSLRKDMY QKMIEVDPEA
781PTEEEKAQRA VTKPRYMQWR ETISSTATLG FRIEGIKKED GTVNRDFKKT KTREQVTEAF
841REFTKGNHNI LIAYRDRLKA IRTTLEVSPF FKCHEVIGSS LLFIHDKKEQ AKVWMIDFGK
901TTPLPEGQTL QHDVPWQEGN REDGYLSGLN NLVDILTEMS QDAPLA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

946 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.