Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ITLN2 / Intelectin 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107702-100
Catalog Number: LS-G107702
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITLN2(ITLN2) (NM_080878), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HL2 | Intelectin-2 | ITLN2 | HL-2 | Endothelial lectin HL-2 | Intelectin 2
- Origin Species
- Human
- SwissProt
- Q8WWU7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLSMLRTMTR LCFLLFFSVA TSGCSAAAAS SLEMLSREFE TCAFSFSSLP RSCKEIKERC61HSAGDGLYFL RTKNGVVYQT FCDMTSGGGG WTLVASVHEN DMRGKCTVGD RWSSQQGNKA121DYPEGDGNWA NYNTFGSAEA ATSDDYKNPG YYDIQAKDLG IWHVPNKSPM QHWRNSALLR181YRTNTGFLQR LGHNLFGIYQ KYPVKYRSGK CWNDNGPAIP VVYDFGDAKK TASYYSPYGQ241REFVAGFVQF RVFNNERAAN ALCAGIKVTG CNTEHHCIGG GGFFPQGKPR QCGDFSAFDW301DGYGTHVKSS CSREITEAAV LLFYRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
325 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.