Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ITIH6 / ITIH5L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103663-100
Catalog Number: LS-G103663
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITIH6 (NM_198510), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_198510
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 143 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGWRYLICV SFLLTILLEL TYQGPPVPAS SSTKLLMTSY SMRSTVVSRY AHTLVTSVLF61NPHAEAHEAI FDLDLPHLAF ISNFTMTINN KVYIAEVKEK HQAKKIYEEA HQQGKTAAHV121GIRDRESEKF RISTSLAAGT EVTFSLAYEE LLQRHQGQYQ LVVSLRPGQL VKRLSIEVTV181SERTGISYVH IPPLRTGRLR TNAHASEVDS PPSTRIERGE TCVRITYCPT LQDQSSISGS241GIMADFLVQY DVVMEDIIGD VQIYDDYFIH YFAPRGLPPM EKNVVFVIDV SSSMFGTKME301QTKTAMNVIL SDLQANDYFN IISFSDTVNV WKAGGSIQAT IQNVHSAKDY LHCMEADGWT361DVNSALLAAA SVLNHSNQEP GRGPSVGRIP LIIFLTDGEP TAGVTTPSVI LSNVRQALGH421RVSLFSLAFG DDADFTLLRR LSLENRGIAR RIYEDTDAAL QLKGLYEEIS MPLLADVRLN481YLGGLVGASP WAVFPNYFGG SELVVAGQVQ PGKQELGIHL AARGPKDQLL VAHHSEGATN541NSQKAFGCPG EPAPNVAHFI RRLWAYVTIG ELLDAHFQAR DTTTRHLLAA KVLNLSLEYN601FVTPLTSLVM VQPKQASEET RRQTSTSAGP DTIMPSSSSR HGLGVSTAQP ALVPKVISPK661SRPVKPKFYL SSTTTASTKK MLSSKELEPL GESPHTLSMP TYPKAKIPAQ QDSGTLAQPT721LRTKPTILVP SNSGTLLPLK PGSLSHQNPD ILPTNSRTQV PPVKPGIPAS PKADTVKCVT781PLHSKPGAPS HPQLGALTSQ APKGLPQSRP GVSTLQVPKY PLHTRPRVPA PKTRNNMPHL841GPGILLSKTP KILLSLKPSA PPHQISTSIS LSKPETPNPH MPQTPLPPRP DRPRPPLPES901LSTFPNTISS STGPSSTTTT SVLGEPLPMP FTPTLPPGRF WHQYDLLPGP QRTRQVLGPS961RPGVPTMSLL NSSRPTPEGS PPNLPILLPS SILPEAISLL LLPEELELLS ESMVESKFVE1021SLNPPAFYTF LTPDEDGSPN WDGNSEEILG GAGGSMESQG SSVGLAKGTL PSIFTFSSSV1081DGDPHFVIQI PHSEEKICFT LNGHPGDLLQ LIEDPKAGLH VSGKLLGAPP RPGHKDQTRT1141YFQIITVTTD KPRAYTITIS RSSISLRGEG TLRLSWDQPA LLKRPQLELY VAAAARLTLR1201LGPYLEFLVL RHRYRHPSTL QLPHLGFYVA NGSGLSPSAR GLIGQFQHAD IRLVTGPMGP1261CLRRHHGPDV PVILGKRLLK DSPRLLPRWA SCWLVKRSHV ELLLGHPYLS YVLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1313 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.