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ITIH5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ITIH5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ITIH5 antibodies →
$725.00each
Catalog Number: LS-G109519-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITIH5 (NM_030569), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1953 | ITI heavy chain H5 | ITI-HC5 | PP14776 | ITIH5
Origin Species
Human
NCBI GenBank
NM_030569
SwissProt
Q86UX2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MLLLLGLCLG LSLCVGSQEE AQSWGHSSEQ DGLRVPRQVR LLQRLKTKPL MTEFSVKSTI
61ISRYAFTTVS CRMLNRASED QDIEFQMQIP AAAFITNFTM LIGDKVYQGE ITEREKKSGD
121RVKEKRNKTT EENGEKGTEI FRASAVIPSK DKAAFFLSYE ELLQRRLGKY EHSISVRPQQ
181LSGRLSVDVN ILESAGIASL EVLPLHNSRQ RGSGRGEDDS GPPPSTVINQ NETFANIIFK
241PTVVQQARIA QNGILGDFII RYDVNREQSI GDIQVLNGYF VHYFAPKDLP PLPKNVVFVL
301DSSASMVGTK LRQTKDALFT ILHDLRPQDR FSIIGFSNRI KVWKDHLISV TPDSIRDGKV
361YIHHMSPTGG TDINGALQRA IRLLNKYVAH SGIGDRSVSL IVFLTDGKPT VGETHTLKIL
421NNTREAARGQ VCIFTIGIGN DVDFRLLEKL SLENCGLTRR VHEEEDAGSQ LIGFYDEIRT
481PLLSDIRIDY PPSSVVQATK TLFPNYFNGS EIIIAGKLVD RKLDHLHVEV TASNSKKFII
541LKTDVPVRPQ KAGKDVTGSP RPGGDGEGDT NHIERLWSYL TTKELLSSWL QSDDEPEKER
601LRQRAQALAV SYRFLTPFTS MKLRGPVPRM DGLEEAHGMS AAMGPEPVVQ SVRGAGTQPG
661PLLKKPYQPR IKISKTSVDG DPHFVVDFPL SRLTVCFNID GQPGDILRLV SDHRDSGVTV
721NGELIGAPAP PNGHKKQRTY LRTITILINK PERSYLEITP SRVILDGGDR LVLPCNQSVV
781VGSWGLEVSV SANANVTVTI QGSIAFVILI HLYKKPAPFQ RHHLGFYIAN SEGLSSNCHG
841LLGQFLNQDA RLTEDPAGPS QNLTHPLLLQ VGEGPEAVLT VKGHQVPVVW KQRKIYNGEE
901QIDCWFARNN AAKLIDGEYK DYLASHPFDT GMDTWPGNVQ GALKLAALKM QVHEGQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

956 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.