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ITIH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ITIH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ITIH2 antibodies →
$725.00each
Catalog Number: LS-G99693-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITIH2 (NM_002216), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IGHEP2 | ITI-HC2 | ITIH2 | H2P | ITI heavy chain H2 | SHAP
NCBI GenBank
NM_002216
SwissProt
P19823

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
106.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKRLTCFFIC FFLSEVSGFE IPINGLSEFV DYEDLVELAP GKFQLVAENR RYQRSLPGES
61EEMMEEVDQV TLYSYKVQST ITSRMATTMI QSKVVNNSPQ PQNVVFDVQI PKGAFISNFS
121MTVDGKTFRS SIKEKTVGRA LYAQARAKGK TAGLVRSSAL DMENFRTEVN VLPGAKVQFE
181LHYQEVKWRK LGSYEHRIYL QPGRLAKHLE VDVWVIEPQG LRFLHVPDTF EGHFDGVPVI
241SKGQQKAHVS FKPTVAQQRI CPSCRETAVD GELVVLYDVK REEKAGELEV FNGYFVHFFA
301PDNLDPIPKN ILFVIDVSGS MWGVKMKQTV EAMKTILDDL RAEDHFSVID FNQNIRTWRN
361DLISATKTQV ADAKRYIEKI QPSGGTNINE ALLRAIFILN EANNLGLLDP NSVSLIILVS
421DGDPTVGELK LSKIQKNVKE NIQDNISLFS LGMGFDVDYD FLKRLSNENH GIAQRIYGNQ
481DTSSQLKKFY NQVSTPLLRN VQFNYPHTSV TDVTQNNFHN YFGGSEIVVA GKFDPAKLDQ
541IESVITATSA NTQLVLETLA QMDDLQDFLS KDKHADPDFT RKLWAYLTIN QLLAERSLAP
601TAAAKRRITR SILQMSLDHH IVTPLTSLVI ENEAGDERML ADAPPQDPSC CSGALYYGSK
661VVPDSTPSWA NPSPTPVISM LAQGSQVLES TPPPHVMRVE NDPHFIIYLP KSQKNICFNI
721DSEPGKILNL VSDPESGIVV NGQLVGAKKP NNGKLSTYFG KLGFYFQSED IKIEISTETI
781TLSHGSSTFS LSWSDTAQVT NQRVQISVKK EKVVTITLDK EMSFSVLLHR VWKKHPVNVD
841FLGIYIPPTN KFSPKAHGLI GQFMQEPKIH IFNERPGKDP EKPEASMEVK GQKLIITRGL
901QKDYRTDLVF GTDVTCWFVH NSGKGFIDGH YKDYFVPQLY SFLKRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

946 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.