Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ITGA9 / Integrin Alpha 9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99691-100
Catalog Number: LS-G99691
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Integrin alpha 9(ITGA9) (NM_002207), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Integrin alpha-9 | Integrin alpha-RLC | ITGA9 | ALPHA-RLC | Integrin Alpha 9 | Integrin | alpha 9 | ITGA4L | RLC
- NCBI GenBank
- NM_002207
- SwissProt
- Q13797
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 114.52 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGPAAPRGA GRLRALLLAL VVAGIPAGAY NLDPQRPVHF QGPADSFFGY AVLEHFHDNT61RWVLVGAPKA DSKYSPSVKS PGAVFKCRVH TNPDRRCTEL DMARGKNRGT SCGKTCREDR121DDEWMGVSLA RQPKADGRVL ACAHRWKNIY YEADHILPHG FCYIIPSNLQ AKGRTLIPCY181EEYKKKYGEE HGSCQAGIAG FFTEELVVMG APGSFYWAGT IKVLNLTDNT YLKLNDEVIM241NRRYTYLGYA VTAGHFSHPS TIDVVGGAPQ DKGIGKVYIF RADRRSGTLI KIFQASGKKM301GSYFGSSLCA VDLNGDGLSD LLVGAPMFSE IRDEGQVTVY INRGNGALEE QLALTGDGAY361NAHFGESIAS LDDLDNDGFP DVAIGAPKED DFAGAVYIYH GDASGIVPQY SMKLSGQKIN421PVLRMFGQSI SGGIDMDGNG YPDVTVGAFM SDSVVLLRAR PVITVDVSIF LPGSINITAP481QCHDGQQPVN CLNVTTCFSF HGKHVPGEIG LNYVLMADVA KKEKGQMPRV YFVLLGETMG541QVTEKLQLTY MEETCRHYVA HVKRRVQDVI SPIVFEAAYS LSEHVTGEEE RELPPLTPVL601RWKKGQKIAQ KNQTVFERNC RSEDCAADLQ LQGKLLLSSM DEKTLYLALG AVKNISLNIS661ISNLGDDAYD ANVSFNVSRE LFFINMWQKE EMGISCELLE SDFLKCSVGF PFMRSKSKYE721FSVIFDTSHL SGEEEVLSFI VTAQSGNTER SESLHDNTLV LMVPLMHEVD TSITGIMSPT781SFVYGESVDA ANFIQLDDLE CHFQPINITL QVYNTGPSTL PGSSVSISFP NRLSSGGAEM841FHVQEMVVGQ EKGNCSFQKN PTPCIIPQEQ ENIFHTIFAF FTKSGRKVLD CEKPGISCLT901AHCNFSALAK EESRTIDIYM LLNTEILKKD SSSVIQFMSR AKVKVDPALR VVEIAHGNPE961EVTVVFEALH NLEPRGYVVG WIIAISLLVG ILIFLLLAVL LWKMGFFRRR YKEIIEAEKN1021RKENEDSWDW VQKNQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1035 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.