Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ITGA11 / Integrin Alpha 11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122245-100
Catalog Number: LS-G122245
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITGA11 (NM_001004439), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Integrin alpha-11 | Integrin | alpha 11 | ITGA11 | HsT18964
- Origin Species
- Human
- NCBI GenBank
- NM_012211
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 133.47 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDLPRGLVVA WALSLWPGFT DTFNMDTRKP RVIPGSRTAF FGYTVQQHDI SGNKWLVVGA61PLETNGYQKT GDVYKCPVIH GNCTKLNLGR VTLSNVSERK DNMRLGLSLA TNPKDNSFLA121CSPLWSHECG SSYYTTGMCS RVNSNFRFSK TVAPALQRCQ TYMDIVIVLD GSNSIYPWVE181VQHFLINILK KFYIGPGQIQ VGVVQYGEDV VHEFHLNDYR SVKDVVEAAS HIEQRGGTET241RTAFGIEFAR SEAFQKGGRK GAKKVMIVIT DGESHDSPDL EKVIQQSERD NVTRYAVAVL301GYYNRRGINP ETFLNEIKYI ASDPDDKHFF NVTDEAALKD IVDALGDRIF SLEGTNKNET361SFGLEMSQTG FSSHVVEDGV LLGAVGAYDW NGAVLKETSA GKVIPLRESY LKEFPEELKN421HGAYLGYTVT SVVSSRQGRV YVAGAPRFNH TGKVILFTMH NNRSLTIHQA MRGQQIGSYF481GSEITSVDID GDGVTDVLLV GAPMYFNEGR ERGKVYVYEL RQNLFVYNGT LKDSHSYQNA541RFGSSIASVR DLNQDSYNDV VVGAPLEDNH AGAIYIFHGF RGSILKTPKQ RITASELATG601LQYFGCSIHG QLDLNEDGLI DLAVGALGNA VILWSRPVVQ INASLHFEPS KINIFHRDCK661RSGRDATCLA AFLCFTPIFL APHFQTTTVG IRYNATMDER RYTPRAHLDE GGDRFTNRAV721LLSSGQELCE RINFHVLDTA DYVKPVTFSV EYSLEDPDHG PMLDDGWPTT LRVSVPFWNG781CNEDEHCVPD LVLDARSDLP TAMEYCQRVL RKPAQDCSAY TLSFDTTVFI IESTRQRVAV841EATLENRGEN AYSTVLNISQ SANLQFASLI QKEDSDGSIE CVNEERRLQK QVCNVSYPFF901RAKAKVAFRL DFEFSKSIFL HHLEIELAAG SDSNERDSTK EDNVAPLRFH LKYEADVLFT961RSSSLSHYEV KPNSSLERYD GIGPPFSCIF RIQNLGLFPI HGMMMKITIP IATRSGNRLL1021KLRDFLTDEA NTSCNIWGNS TEYRPTPVEE DLRRAPQLNH SNSDVVSINC NIRLVPNQEI1081NFHLLGNLWL RSLKALKYKS MKIMVNAALQ RQFHSPFIFR EEDPSRQIVF EISKQEDWQV1141PIWIIVGSTL GGLLLLALLV LALWKLGFFR SARRRREPGL DPTPKVLETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1188 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.