Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ITFG1 / TIP Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109403-100
Catalog Number: LS-G109403
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDA08(ITFG1) (NM_030790), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDA08 | ITFG1 | Protein TIP | TIP
- Origin Species
- Human
- NCBI GenBank
- NM_030790
- SwissProt
- Q8TB96
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAGRLPSS WALFSPLLAG LALLGVGPVP ARALHNVTAE LFGAEAWGTL AAFGDLNSDK61QTDLFVLRER NDLIVFLADQ NAPYFKPKVK VSFKNHSALI TSVVPGDYDG DSQMDVLLTY121LPKNYAKSEL GAVIFWGQNQ TLDPNNMTIL NRTFQDEPLI MDFNGDLIPD IFGITNESNQ181PQILLGGNLS WHPALTTTSK MRIPHSHAFI DLTEDFTADL FLTTLNATTS TFQFEIWENL241DGNFSVSTIL EKPQNMMVVG QSAFADFDGD GHMDHLLPGC EDKNCQKSTI YLVRSGMKQW301VPVLQDFSNK GTLWGFVPFV DEQQPTEIPI PITLHIGDYN MDGYPDALVI LKNTSGSNQQ361AFLLENVPCN NASCEEARRM FKVYWELTDL NQIKDAMVAT FFDIYEDGIL DIVVLSKGYT421KNDFAIHTLK NNFEADAYFV KVIVLSGLCS NDCPRKITPF GVNQPGPYIM YTTVDANGYL481KNGSAGQLSQ SAHLALQLPY NVLGLGRSAN FLDHLYVGIP RPSGEKSIRK QEWTAIIPNS541QLIVIPYPHN VPRSWSAKLY LTPSNIVLLT AIALIGVCVF ILAIIGILHW QEKKADDREK601RQEAHRFHFD AMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
612 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.