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ISM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ISM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103384-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ISM2 (NM_199296), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRALRDRAGL LLCVLLLAAL LEAALGLPVK KPRLRGPRPG SLTRLAEVSA SPDPRPLKEE
61EEAPLLPRTH LQAEPHQHGC WTVTEPAAMT PGNATPPRTP EVTPLRLELQ KLPGLANTTL
121STPNPDTQAS ASPDPRPLRE EEEARLLPRT HLQAELHQHG CWTVTEPAAL TPGNATPPRT
181QEVTPLLLEL QKLPELVHAT LSTPNPDNQV TIKVVEDPQA EVSIDLLAEP SNPPPQDTLS
241WLPALWSFLW GDYKGEEKDR APGEKGEEKE EDEDYPSEDI EGEDQEDKEE DEEEQALWFN
301GTTDNWDQGW LAPGDWVFKD SVSYDYEPQK EWSPWSPCSG NCSTGKQQRT RPCGYGCTAT
361ETRTCDLPSC PGTEDKDTLG LPSEEWKLLA RNATDMHDQD VDSCEKWLNC KSDFLIKYLS
421QMLRDLPSCP CAYPLEAMDS PVSLQDEHQG RSFRWRDASG PRERLDIYQP TARFCLRSML
481SGESSTLAAQ HCCYDEDSRL LTRGKGAGMP NLISTDFSPK LHFKFDTTPW ILCKGDWSRL
541HAVLPPNNGR ACTDNPLEEE YLAQLQEAKE Y
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

571 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.