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IRX5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IRX5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G115704-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IRX5 (NM_005853), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IRX2A | Homeodomain protein IRX-2A | Iroquois homeobox 5 | Iroquois homeobox protein 5 | IRX5 | HMMS | Homeodomain protein IRXB2 | IRX-2a | IRXB2
Origin Species
Human
NCBI GenBank
NM_005853
SwissProt
P78411

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSYPQGYLYQ PSASLALYSC PAYSTSVISG PRTDELGRSS SGSAFSPYAG STAFTAPSPG
61YNSHLQYGAD PAAAAAAAFS SYVGSPYDHT PGMAGSLGYH PYAAPLGSYP YGDPAYRKNA
121TRDATATLKA WLNEHRKNPY PTKGEKIMLA IITKMTLTQV STWFANARRR LKKENKMTWT
181PRNRSEDEEE EENIDLEKND EDEPQKPEDK GDPEGPEAGG AEQKAASGCE RLQGPPTPAG
241KETEGSLSDS DFKEPPSEGR LDALQGPPRT GGPSPAGPAA ARLAEDPAPH YPAGAPAPGP
301HPAAGEVPPG PGGPSVIHSP PPPPPPAVLA KPKLWSLAEI ATSSDKVKDG GGGNEGSPCP
361PCPGPIAGQA LGGSRASPAP APSRSPSAQC PFPGGTVLSR PLYYTAPFYP GYTNYGSFGH
421LHGHPGPGPG PTTGPGSHFN GLNQTVLNRA DALAKDPKML RSQSQLDLCK DSPYELKKGM
481SDI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

483 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.