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IRX4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IRX4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112698-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IRX4 (NM_016358), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Iroquois homeobox protein 4 | IRX4 | Homeodomain protein IRXA3 | Iroquois homeobox 4 | IRXA3
Origin Species
Human
NCBI GenBank
NM_016358
SwissProt
P78413

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSYPQFGYPY SSAPQFLMAT NSLSTCCESG GRTLADSGPA ASAQAPVYCP VYESRLLATA
61RHELNSAAAL GVYGGPYGGS QGYGNYVTYG SEASAFYSLN SFDSKDGSGS AHGGLAPATA
121AYYPYEPALG QYPYDRYGTM DSGTRRKNAT RETTSTLKAW LQEHRKNPYP TKGEKIMLAI
181ITKMTLTQVS TWFANARRRL KKENKMTWPP RNKCADEKRP YAEGEEEEGG EEEAREEPLK
241SSKNAEPVGK EEKELELSDL DDFDPLEAEP PACELKPPFH SLDGGLERVP AAPDGPVKEA
301SGALRMSLAA GGGAALDEDL ERARSCLRSA AAGPEPLPGA EGGPQVCEAK LGFVPAGASA
361GLEAKPRIWS LAHTATAAAA AATSLSQTEF PSCMLKRQGP AAPAAVSSAP ATSPSVALPH
421SGALDRHQDS PVTSLRNWVD GVFHDPILRH STLNQAWATA KGALLDPGPL GRSLGAGANV
481LTAPLARAFP PAVPQDAPAA GAARELLALP KAGGKPFCA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

519 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.