LSBio
IRGQ Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IRGQ Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IRGQ antibodies →
$725.00each
Catalog Number: LS-G122012-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IRGQ (NM_001007561), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IRGQ1 | FKSG27 | IRGQ
Origin Species
Human
NCBI GenBank
NM_001007561
SwissProt
Q8WZA9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPPPQGDVTA LFLGPPGLGK SALIAALCDK DVETLEAPEG RPDSGVPSLR AAGPGLFLGE
61LSCPPAAPGP WAAEANVLVL VLPGPEGNGE PLAPALGEAA LAALARGTPL LAVRNLRPGD
121SQTAAQARDQ TAALLNSAGL GAADLFVLPA NCGSSDGCEE LERLRAALQS QAEALRRLLP
181PAQDGFEVLG AAELEAVREA FETGGLEAAL SWVRSGLERL GSARLDLAVA GKADVGLVVD
241MLLGLDPGDP GAAPASVPTA PTPFPAPERP NVVLWTVPLG HTGTATTAAA ASHPTHYDAL
301ILVTPGAPTE KDWAQVQALL LPDAPLVCVR TDGEGEDPEC LGEGKMENPK GESLKNAGGG
361GLENALSKGR EKCSAGSQKA GSGEGPGKAG SEGLQQVVGM KKSGGGDSER AAALSPEDET
421WEVLEEAPPP VFPLRPGGLP GLCEWLRRAL PPAQAGALLL ALPPASPSAA RTKAAALRAG
481AWRPALLASL AAAAAPLPGL GWACDVALLR GQLAEWRRGL GLEPTALARR ERALGLASGE
541LAARAHFPGP VTRAEVEARL GAWAGEGTAG GAALGALSFL WPAGGAAATG GLGYRAAHGV
601LLQALDEMRA DAEAVLAPPE PAQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

623 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.