Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IRGC1 / CINEMA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111420-100
Catalog Number: LS-G111420
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IRGC (NM_019612), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CINEMA | Iigp5 | IFGGE | Interferon inducible GTPase 5 | IRGC1 | Interferon-inducible GTPase 5 | R30953_1 | IRGC
- Origin Species
- Human
- NCBI GenBank
- NM_019612
- SwissProt
- Q6NXR0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATSKLPVVP GEEENTILMA KERLEALRTA FESGDLPQAA SHLQELLAST ESIRLEVGVT61GESGAGKSSL INALRGLEAE DPGAALTGVM ETTMQPSPYP HPQFPDVTLW DLPGAGSPGC121PADKYLKQVD FSRYDFFLLV SPRRCGAVET RLAAEILCQG KKFYFVRTKV DEDLAATRTQ181RPSGFREAAV LQEIRDHCAE RLREAGVADP RIFLVSNLSP ARYDFPTLVS TWEHDLPSHR241RHAGLLSLPD ISLEALQKKK AMLQEQVLKT ALVLGVIQAL PVPGLAAAYD DALLIHSLRG301YHRSFGLDDD SLAKLAEQVG KQAGDLRSVI RSPLANEVSP ETVLRLYSQS SDGAMRVARA361FERGIPVFGT LVAGGISFGA VYTMLQGCLN EMAEDAQRVR IKALEDDEPQ PEVSLEVASD421NGVEKGGSGE GGGEEAPLST CRKLGLLLKY ILDSWKKHDS EEKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
463 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.