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IRF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IRF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G116945-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IRF7 (NM_004031), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Interferon regulatory factor 7 | IRF-7 | IRF7A | IRF-7H | IRF7
Origin Species
Human
NCBI GenBank
NM_004031
SwissProt
Q92985

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPVPERPAAG PDSPRPGTRR AAPRVLFGEW LLGEISSGCY EGLQWLDEAR TCFRVPWKHF
61ARKDLSEADA RIFKAWAVAR GRWPPSSRGG GPPPEAETAE RAGWKTNFRC ALRSTRRFVM
121LRDNSGDPAD PHKVYALSRE LCWREGPGTD QTEAEAPAAV PPPQGGPPGP FLAHTHAGLQ
181APGPLPAPAG DEGDLLLQAV QQSCLADHLL TASWGADPVP TKAPGEGQEG LPLTGACAGG
241PGLPAGELYG WAVETTPSPG PQPAALTTGE AAAPESPHQA EPYLSPSPSA CTAVQEPSPG
301ALDVTIMYKG RTVLQKVVGH PSCTFLYGPP DPAVRATDPQ QVAFPSPAEL PDQKQLRYTE
361ELLRHVAPGL HLELRGPQLW ARRMGKCKVY WEVGGPPGSA SPSTPACLLP RNCDTPIFDF
421RVFFRELVEF RARQRRGSPR YTIYLGFGQD LSAGRPKEKS LVLVKLEPWL CRVHLEGTQR
481EGVSSLDSSS LSLCLSSANS LYDDIECFLM ELEQPA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

516 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.