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IRF2BP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IRF2BP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IRF2BP2 antibodies →
$725.00each
Catalog Number: LS-G104052-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IRF2BP2 (NM_182972), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IRF2BP2 | IRF-2-binding protein 2 | IRF-2BP2
Origin Species
Human
SwissProt
Q7Z5L9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAVAVAAA SRRQSCYLCD LPRMPWAMIW DFTEPVCRGC VNYEGADRVE FVIETARQLK
61RAHGCFPEGR SPPGAAASAA AKPPPLSAKD ILLQQQQQLG HGGPEAAPRA PQALERYPLA
121AAAERPPRLG SDFGSSRPAA SLAQPPTPQP PPVNGILVPN GFSKLEEPPE LNRQSPNPRR
181GHAVPPTLVP LMNGSATPLP TALGLGGRAA ASLAAVSGTA AASLGSAQPT DLGAHKRPAS
241VSSSAAVEHE QREAAAKEKQ PPPPAHRGPA DSLSTAAGAA ELSAEGAGKS RGSGEQDWVN
301RPKTVRDTLL ALHQHGHSGP FESKFKKEPA LTAGRLLGFE ANGANGSKAV ARTARKRKPS
361PEPEGEVGPP KINGEAQPWL STSTEGLKIP MTPTSSFVSP PPPTASPHSN RTTPPEAAQN
421GQSPMAALIL VADNAGGSHA SKDANQVHST TRRNSNSPPS PSSMNQRRLG PREVGGQGAG
481NTGGLEPVHP ASLPDSSLAT SAPLCCTLCH ERLEDTHFVQ CPSVPSHKFC FPCSRQSIKQ
541QGASGEVYCP SGEKCPLVGS NVPWAFMQGE IATILAGDVK VKKERDS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

587 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.