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IRF2BP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IRF2BP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IRF2BP1 antibodies →
$504.00each
Catalog Number: LS-G113080-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IRF2BP1 (NM_015649), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434M154 | IRF2BP1 | IRF-2-binding protein 1 | IRF-2BP1
Origin Species
Human
NCBI GenBank
AL080159
SwissProt
Q8IU81

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASVQASRRQ WCYLCDLPKM PWAMVWDFSE AVCRGCVNFE GADRIELLID AARQLKRSHV
61LPEGRSPGPP ALKHPATKDL AAAAAQGPQL PPPQAQPQPS GTGGGVSGQD RYDRATSSGR
121LPLPSPALEY TLGSRLANGL GREEAVAEGA RRALLGSMPG LMPPGLLAAA VSGLGSRGLT
181LAPGLSPARP LFGSDFEKEK QQRNADCLAE LNEAMRGRAE EWHGRPKAVR EQLLALSACA
241PFNVRFKKDH GLVGRVFAFD ATARPPGYEF ELKLFTEYPC GSGNVYAGVL AVARQMFHDA
301LREPGKALAS SGFKYLEYER RHGSGEWRQL GELLTDGVRS FREPAPAEAL PQQYPEPAPA
361ALCGPPPRAP SRNLAPTPRR RKASPEPEGE AAGKMTTEEQ QQRHWVAPGG PYSAETPGVP
421SPIAALKNVA EALGHSPKDP GGGGGPVRAG GASPAASSTA QPPTQHRLVA RNGEAEVSPT
481AGAEAVSGGG SGTGATPGAP LCCTLCRERL EDTHFVQCPS VPGHKFCFPC SREFIKAQGP
541AGEVYCPSGD KCPLVGSSVP WAFMQGEIAT ILAGDIKVKK ERDP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

584 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.