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IQUB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQUB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQUB antibodies →
$725.00each
Catalog Number: LS-G104591-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQUB (NM_178827), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IQUB
Origin Species
Human
SwissProt
Q8NA54

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSNRQEKYEA QNIVNSTEES DDAFDTVTIP VPSEEPQESD QTEEHESGIE QFSESHAIHV
61EEQSDQSFSS LEPDNEQLME EVISPRQVSY TPQHHEKQYA MQRPNDDSLA FLDKIKSVKE
121SLQESVEDSL ATVKVVLIPV GQEIVIPFKV DTILKYLKDH FSHLLGIPHS VLQIRYSGKI
181LKNNETLVQH GVKPQEIVQV EIFSTNPDLY PVRRIDGLTD VSQIITVTVQ TGLDQYQQVP
241VEIVKSDFHK PFLGGFRHKV TGVEYHNAGT QTVPKRIPER LSIFCRDTQT VFQKKNLQQT
301TNTTSTQMTN IGVYVSNMTD KLVTPGKYFS AAEYHAQRLK AVIVIQTYYR QWHAKIFVEN
361LRRQKSLRLE WETQQELRKI REKEEWIKLD YHRRHNPKTN EDFEFLYNAL EFWRQEELTR
421INQSFTGAER KAALCELLEK ETQIIASIGR HRYIAYMANQ EAAIQAFLDK CSAPKIWRTP
481NGKTIEMDTQ FTIRARELQN IYKCIMLKNI SQDERLDVLL TLKHTVKEHE CKLTQEILEL
541IDREVDLMMR GVKHHNLEGL RKRIATLFFH YIKTPLFNPE VAKYLKVPQD PLKFYKKIYF
601CHSCQLYLPS TEFSVSSTSR RIYRCRNCIN LQNEAQKRES FLKYKCLLQQ LYYTEADYED
661DSKIAFLMQL QDIQYLTENI WASQSVLSAC DNLSDLVMVR WNKSLEWSPW NCILLTKDEA
721AAHLKLTSIE EGYERSFIHK IKHKHILAKN YFSQVPVLAS FILDDGEIDE IRWKYHSDTT
781PKIIESQRPP H
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

791 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.