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IQSEC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQSEC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQSEC3 antibodies →
$725.00each
Catalog Number: LS-G130401-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQSEC3 (NM_001170738), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IQ motif and Sec7 domain 3 | IQSEC3 | KIAA1110
Origin Species
Human
NCBI GenBank
AA056734

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
128.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESLLENPVR AVLYLKELTA IVQNQQSLIH TQRERIDELE RRLDELSAEN RSLWEHQQLL
61QAQPPPGLVP PSSAPLPAAP ATAPAAAARA QEPLQDQGQR SAAAPHPAPD RPPRQHHGQL
121LEQPQRGPGS RAHTPQSPHK HLGTQGAVTD KEKERPPSCC AAAGALLQHK SPSALGKGVL
181SRRPENETVL HQFCCPAADA CSDLASQSDG SCTQAGGGME DSVVAAAAVA AGRPSAHAPK
241AQAQELQEEE ERPGAGAASP RAGPQHKASP GRQQPALATA LCPHAPAASD YELSLDLKNK
301QIEMLEHKYG GHLVSRRAAC TIQTAFRQYQ LSKNFEKIRN SLLESRLPRR ISLRKVRSPT
361AESLAAEKAL MEGYGLVGLP LVRSPSLPPT FAGTLTELED SFTEQVQSLA KSIDDALSTW
421SLKTMCSLRE SGAYQLHQAL QAAAGPPGLE AEGRAPESAG PGPGDDAAET PGLPPAHSGT
481LMMAFRDVTV QIANQNISVS SSTALSVANC LGAQTVQAPA EPAAGKAEQG ETSGREAPEA
541PAVGREDASA EDSCAEAAAS GAADGATAPK TEEEEEEEET AEVGRGAEAE AGDLEQLSSS
601STSTKSAKSG SEASASASKD ALQAMILSLP RYHCENPASC KSPTLSTDTL RKRLYRIGLN
661LFNINPDKGI QFLISRGFIP DTPIGVAHFL LQRKGLSRQM IGEFLGNSKK QFNRDVLDCV
721VDEMDFSSME LDEALRKFQA HIRVQGEAQK VERLIEAFSQ RYCMCNPEVV QQFHNPDTIF
781ILAFAIILLN TDMYSPNIKP DRKMMLEDFI RNLRGVDDGA DIPRELVVGI YERIQQKELK
841SNEDHVTYVT KVEKSIVGMK TVLSVPHRRL VCCSRLFEVT DVNKLQKQAA HQREVFLFND
901LLVILKLCPK KKSSSTYTFC KSVGLLGMQF QLFENEYYSH GITLVTPLSG SEKKQVLHFC
961ALGSDEMQKF VEDLKESIAE VTELEQIRIE WELEKQQGTK TLSFKPCGAQ GDPQSKQGSP
1021TAKREAALRE RPAESTVEVS IHNRLQTSQH NSGLGAERGA PVPPPDLQPS PPRQQTPPLP
1081PPPPTPPGTL VQCQQIVKVI VLDKPCLARM EPLLSQALSC YTSSSSDSCG STPLGGPGSP
1141VKVTHQPPLP PPPPPYNHPH QFCPPGSLLH GHRYSSGSRS LV
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1182 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.