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IQSEC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQSEC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQSEC1 antibodies →
$725.00each
Catalog Number: LS-G125254-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQSEC1 (NM_001134382), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARF-GEP100 | ARFGEP100 | Brefeldin A-resistant ARF-GEF2 | BRAG2 | IQ motif and Sec7 domain 1 | IQSEC1 | KIAA0763 | GEP100
Origin Species
Human
NCBI GenBank
NM_014869
SwissProt
Q6DN90

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MACRRRYFVE GEAPSSETGT SLDSPSAYPQ GPLVPGSSLS PDHYEHTSVG AYGLYSGPPG
61QQQRTRRPKL QHSTSILRKQ AEEEAIKRSR SLSESYELSS DLQDKQVEML ERKYGGRLVT
121RHAARTIQTA FRQYQMNKNF ERLRSSMSEN RMSRRIVLSN MRMQFSFEGP EKVHSSYFEG
181KQVSVTNDGS QLGALVSPEC GDLSEPTTLK SPAPSSDFAD AITELEDAFS RQVKSLAESI
241DDALNCRSLH TEEAPALDAA RARDTEPQTA LHGMDHRKLD EMTASYSDVT LYIDEEELSP
301PLPLSQAGDR PSSTESDLRL RAGGAAPDYW ALAHKEDKAD TDTSCRSTPS LERQEQRLRV
361EHLPLLTIEP PSDSSVDLSD RSERGSLKRQ SAYERSLGGQ QGSPKHGPHS GAPKSLPREE
421PELRPRPPRP LDSHLAINGS ANRQSKSESD YSDGDNDSIN STSNSNDTIN CSSESSSRDS
481LREQTLSKQT YHKEARNSWD SPAFSNDVIR KRHYRIGLNL FNKKPEKGVQ YLIERGFVPD
541TPVGVAHFLL QRKGLSRQMI GEFLGNRQKQ FNRDVLDCVV DEMDFSTMEL DEALRKFQAH
601IRVQGEAQKV ERLIEAFSQR YCICNPGVVR QFRNPDTIFI LAFAIILLNT DMYSPNVKPE
661RKMKLEDFIK NLRGVDDGED IPREMLMGIY ERIRKRELKT NEDHVSQVQK VEKLIVGKKP
721IGSLHPGLGC VLSLPHRRLV CYCRLFEVPD PNKPQKLGLH QREIFLFNDL LVVTKIFQKK
781KNSVTYSFRQ SFSLYGMQVL LFENQYYPNG IRLTSSVPGA DIKVLINFNA PNPQDRKKFT
841DDLRESIAEV QEMEKHRIES ELEKQKGVVR PSMSQCSSLK KESGNGTLSR ACLDDSYASG
901EGLKRSALSS SLRDLSEAGK RGRRSSAGSL ESNVEGSIIS SPHMRRRATS TRECPSRPHQ
961TMPNSSSLLG SLFGSKRGKP PPQAHLPSAP ALPPPHPPVV LPHLQHSVAG HHLGPPEGLP
1021QAAMHGHHTQ YCHMQNPPPY HHHHHHHPPQ HIQHAHQYHH GPHGGHPAYG AHAHGHPPLP
1081SAHVGHTVHH HGQPPAPPPP TSSKAKPSGI STIV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1114 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.