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IQGAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQGAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQGAP3 antibodies →
$725.00each
Catalog Number: LS-G104726-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQGAP3 (NM_178229), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IQGAP3
Origin Species
Human
SwissProt
Q86VI3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
184.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERRAAGPGW AAYERLTAEE MDEQRRQNVA YQYLCRLEEA KRWMEACLKE ELPSPVELEE
61SLRNGVLLAK LGHCFAPSVV PLKKIYDVEQ LRYQATGLHF RHTDNINFWL SAIAHIGLPS
121TFFPETTDIY DKKNMPRVVY CIHALSLFLF RLGLAPQIHD LYGKVKFTAE ELSNMASELA
181KYGLQLPAFS KIGGILANEL SVDEAAVHAA VLAINEAVER GVVEDTLAAL QNPSALLENL
241REPLAAVYQE MLAQAKMEKA ANARNHDDRE SQDIYDHYLT QAEIQGNINH VNVHGALEVV
301DDALERQSPE ALLKALQDPA LALRGVRRDF ADWYLEQLNS DREQKAQELG LVELLEKEEV
361QAGVAAANTK GDQEQAMLHA VQRINKAIRR GVAADTVKEL MCPEAQLPPV YPVASSMYQL
421ELAVLQQQQG ELGQEELFVA VEMLSAVVLI NRALEARDAS GFWSSLVNPA TGLAEVEGEN
481AQRYFDALLK LRQERGMGED FLSWNDLQAT VSQVNAQTQE ETDRVLAVSL INEALDKGSP
541EKTLSALLLP AAGLDDVSLP VAPRYHLLLV AAKRQKAQVT GDPGAVLWLE EIRQGVVRAN
601QDTNTAQRMA LGVAAINQAI KEGKAAQTER VLRNPAVALR GVVPDCANGY QRALESAMAK
661KQRPADTAFW VQHDMKDGTA YYFHLQTFQG IWEQPPGCPL NTSHLTREEI QSAVTKVTAA
721YDRQQLWKAN VGFVIQLQAR LRGFLVRQKF AEHSHFLRTW LPAVIKIQAH WRGYRQRKIY
781LEWLQYFKAN LDAIIKIQAW ARMWAARRQY LRRLHYFQKN VNSIVKIQAF FRARKAQDDY
841RILVHAPHPP LSVVRRFAHL LNQSQQDFLA EAELLKLQEE VVRKIRSNQQ LEQDLNIMDI
901KIGLLVKNRI TLQEVVSHCK KLTKRNKEQL SDMMVLDKQK GLKSLSKEKR QKLEAYQHLF
961YLLQTQPIYL AKLIFQMPQN KTTKFMEAVI FSLYNYASSR REAYLLLQLF KTALQEEIKS
1021KVEQPQDVVT GNPTVVRLVV RFYRNGRGQS ALQEILGKVI QDVLEDKVLS VHTDPVHLYK
1081NWINQTEAQT GQRSHLPYDV TPEQALSHPE VQRRLDIALR NLLAMTDKFL LAITSSVDQI
1141PYGMRYVAKV LKATLAEKFP DATDSEVYKV VGNLLYYRFL NPAVVAPDAF DIVAMAAGGA
1201LAAPQRHALG AVAQLLQHAA AGKAFSGQSQ HLRVLNDYLE ETHLKFRKFI HRACQVPEPE
1261ERFAVDEYSD MVAVAKPMVY ITVGELVNTH RLLLEHQDCI APDHQDPLHE LLEDLGELPT
1321IPDLIGESIA ADGHTDLSKL EVSLTLTNKF EGLEADADDS NTRSLLLSTK QLLADIIQFH
1381PGDTLKEILS LSASREQEAA HKQLMSRRQA CTAQTPEPLR RHRSLTAHSL LPLAEKQRRV
1441LRNLRRLEAL GLVSARNGYQ GLVDELAKDI RNQHRHRHRR KAELVKLQAT LQGLSTKTTF
1501YEEQGDYYSQ YIRACLDHLA PDSKSSGKGK KQPSLHYTAA QLLEKGVLVE IEDLPASHFR
1561NVIFDITPGD EAGKFEVNAK FLGVDMERFQ LHYQDLLQLQ YEGVAVMKLF NKAKVNVNLL
1621IFLLNKKFLR K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1631 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.