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IQGAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQGAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQGAP1 antibodies →
$725.00each
Catalog Number: LS-G117038-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQGAP1 (NM_003870), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HUMORFA01 | IQGAP1 | p195 | KIAA0051 | RasGAP-like with IQ motifs | SAR1
Origin Species
Human
NCBI GenBank
NM_003870
SwissProt
P46940

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
189.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAADEVDGL GVARPHYGSV LDNERLTAEE MDERRRQNVA YEYLCHLEEA KRWMEACLGE
61DLPPTTELEE GLRNGVYLAK LGNFFSPKVV SLKKIYDREQ TRYKATGLHF RHTDNVIQWL
121NAMDEIGLPK IFYPETTDIY DRKNMPRCIY CIHALSLYLF KLGLAPQIQD LYGKVDFTEE
181EINNMKTELE KYGIQMPAFS KIGGILANEL SVDEAALHAA VIAINEAIDR RIPADTFAAL
241KNPNAMLVNL EEPLASTYQD ILYQAKQDKM TNAKNRTENS ERERDVYEEL LTQAEIQGNI
301NKVNTFSALA NIDLALEQGD ALALFRALQS PALGLRGLQQ QNSDWYLKQL LSDKQQKRQS
361GQTDPLQKEE LQSGVDAANS AAQQYQRRLA AVALINAAIQ KGVAEKTVLE LMNPEAQLPQ
421VYPFAADLYQ KELATLQRQS PEHNLTHPEL SVAVEMLSSV ALINRALESG DVNTVWKQLS
481SSVTGLTNIE EENCQRYLDE LMKLKAQAHA ENNEFITWND IQACVDHVNL VVQEEHERIL
541AIGLINEALD EGDAQKTLQA LQIPAAKLEG VLAEVAQHYQ DTLIRAKREK AQEIQDESAV
601LWLDEIQGGI WQSNKDTQEA QKFALGIFAI NEAVESGDVG KTLSALRSPD VGLYGVIPEC
661GETYHSDLAE AKKKKLAVGD NNSKWVKHWV KGGYYYYHNL ETQEGGWDEP PNFVQNSMQL
721SREEIQSSIS GVTAAYNREQ LWLANEGLIT RLQARCRGYL VRQEFRSRMN FLKKQIPAIT
781CIQSQWRGYK QKKAYQDRLA YLRSHKDEVV KIQSLARMHQ ARKRYRDRLQ YFRDHINDII
841KIQAFIRANK ARDDYKTLIN AEDPPMVVVR KFVHLLDQSD QDFQEELDLM KMREEVITLI
901RSNQQLENDL NLMDIKIGLL VKNKITLQDV VSHSKKLTKK NKEQLSDMMM INKQKGGLKA
961LSKEKREKLE AYQHLFYLLQ TNPTYLAKLI FQMPQNKSTK FMDSVIFTLY NYASNQREEY
1021LLLRLFKTAL QEEIKSKVDQ IQEIVTGNPT VIKMVVSFNR GARGQNALRQ ILAPVVKEIM
1081DDKSLNIKTD PVDIYKSWVN QMESQTGEAS KLPYDVTPEQ ALAHEEVKTR LDSSIRNMRA
1141VTDKFLSAIV SSVDKIPYGM RFIAKVLKDS LHEKFPDAGE DELLKIIGNL LYYRYMNPAI
1201VAPDAFDIID LSAGGQLTTD QRRNLGSIAK MLQHAASNKM FLGDNAHLSI INEYLSQSYQ
1261KFRRFFQTAC DVPELQDKFN VDEYSDLVTL TKPVIYISIG EIINTHTLLL DHQDAIAPEH
1321NDPIHELLDD LGEVPTIESL IGESSGNLND PNKEALAKTE VSLTLTNKFD VPGDENAEMD
1381ARTILLNTKR LIVDVIRFQP GETLTEILET PATSEQEAEH QRAMQRRAIR DAKTPDKMKK
1441SKSVKEDSNL TLQEKKEKIQ TGLKKLTELG TVDPKNKYQE LINDIARDIR NQRRYRQRRK
1501AELVKLQQTY AALNSKATFY GEQVDYYKSY IKTCLDNLAS KGKVSKKPRE MKGKKSKKIS
1561LKYTAARLHE KGVLLEIEDL QVNQFKNVIF EISPTEEVGD FEVKAKFMGV QMETFMLHYQ
1621DLLQLQYEGV AVMKLFDRAK VNVNLLIFLL NKKFYGK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1657 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.