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IQCA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQCA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQCA1 antibodies →
$504.00each
Catalog Number: LS-G109813-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQCA1 (NM_024726), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IQCA | IQCA1
Origin Species
Human
NCBI GenBank
NM_024726
SwissProt
Q86XH1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MSNAMYNKMW HQTQEALGAL LDKEPQKMIE PQRNQVFIFQ TLATFYVKYV QIFRNLENVY
61DQFVHPQKRI LIRKVLDGVM GRILELKNEM VELELTEFHY FDDILQDLKL APQQLDIPIP
121KYFLKEKLEV IKGREKILAQ ILADSGIDTS DMKYPVKSIP FDEAVKLIQI AERARQGRLR
181ALFMKQIYLQ EYRAKQSKML GKKVTDTWAA ALRIQKVWRR FHQRKETEKL REEEMIFLGM
241NPPPLFNEVS ATVIQAEKVD RLRNEVQIKH EEDYREALVT IKNDLKLIEG VDIKENLQDQ
301IRHWFIECRN LTGTFPDYPD VEEGGSAIIF SDKTIQQVIE DIIANQEEEE KNKKKKKKKE
361KQPKKAKKQK KGTKEKNKEE DEKWKMSPSL FLPAMKEGCN AYKEIWMKKD ESWNFSQDYD
421PELIKEEKRK ELQSEIRIQV DELMRQELKN LKLAVDRERE RPVKAGKKKD KKGKKGKKKE
481KKAKKDKDLT ADRTIESLYK ELVEEGLLIQ ALKVNLSDYI GEYSYLGTTL RQVSIEPMPS
541LLDVRQLITL YGIWPLGSAA VHEKAPLVKS LLLAGPSGVG KKMLVHAICT ETGANLFNLS
601SSNIAGKYPG KNGLQMMLHA VFKVARQLQP SVVWIEDTEK TFYKKVPNAE KMNEPKRLKK
661HLPQILKLLK PDDRILIVGT TRRPFDAELQ SFCKVYQKII LVPRPDYASR YVLWKQIIER
721NGGVLTSALN VSCLAKVTDG FTQGHIVEVV KGVLTDQRIR RQIHKPLTAV EFITAITSMN
781PVYKEEEESF KNWYAKTPLG KKRALAITGG STEKAKDKGK RK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

822 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.