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IPPK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IPPK Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IPPK antibodies →
$504.00each
Catalog Number: LS-G101821-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IPPK (NM_022755), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA476B13.1 (novel protein) | BA476B13.1 | C9orf12 | IP5K | IPPK | INSP5K2 | IPK1 homolog | Ins(1 | 3 | 4 | 5 | 6)P5 2-kinase | InsP5 2-kinase | IPK1
Origin Species
Human
NCBI GenBank
NM_022755
SwissProt
Q9H8X2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEGKMDENE WGYHGEGNKS LVVAHAQRCV VLRFLKFPPN RKKTSEEIFQ HLQNIVDFGK
61NVMKEFLGEN YVHYGEVVQL PLEFVKQLCL KIQSERPESR CDKDLDTLSG YAMCLPNLTR
121LQTYRFAEHR PILCVEIKPK CGFIPFSSDV THEMKHKVCR YCMHQHLKVA TGKWKQISKY
181CPLDLYSGNK QRMHFALKSL LQEAQNNLKI FKNGELIYGC KDARSPVADW SELAHHLKPF
241FFPSNGLASG PHCTRAVIRE LVHVITRVLL SGSDKGRAGT LSPGLGPQGP RVCEASPFSR
301SLRCQGKNTP ERSGLPKGCL LYKTLQVQML DLLDIEGLYP LYNRVERYLE EFPEERKTLQ
361IDGPYDEAFY QKLLDLSTED DGTVAFALTK VQQYRVAMTA KDCSIMIALS PCLQDASSDQ
421RPVVPSSRSR FAFSVSVLDL DLKPYESIPH QYKLDGKIVN YYSKTVRAKD NAVMSTRFKE
481SEDCTLVLHK V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

491 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.