Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IPO13 / LGL2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101244-100
Catalog Number: LS-G101244
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Importin 13(IPO13) (NM_014652), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IMP13 | Importin-13 | Karyopherin 13 | IPO13 | Late gestation lung 2 | KIAA0724 | Ran binding protein 13 | Ran-binding protein 13 | Importin 13 | KAP13 | Karyopherin-13 | LGL2 | RANBP13
- Origin Species
- Human
- NCBI GenBank
- NM_014652
- SwissProt
- O94829
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 108 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MERREEQPGA AGAGAAPALD FTVENVEKAL HQLYYDPNIE NKNLAQKWLM QAQVSPQAWH61FSWQLLQPDK VPEIQYFGAS ALHIKISRYW SDIPTDQYES LKAQLFTQIT RFASGSKIVL121TRLCVALASL ALSMMPDAWP CAVADMVRLF QAEDSPVDGQ GRCLALLELL TVLPEEFQTS181RLPQYRKGLV RTSLAVECGA VFPLLEQLLQ QPSSPSCVRQ KVLKCFSSWV QLEVPLQDCE241ALIQAAFAAL QDSELFDSSV EAIVNAISQP DAQRYVNTLL KLIPLVLGLQ EQLRQAVQNG301DMETSHGICR IAVALGENHS RALLDQVEHW QSFLALVNMI MFCTGIPGHY PVNETTSSLT361LTFWYTLQDD ILSFEAEKQA VYQQVYRPVY FQLVDVLLHK AQFPSDEEYG FWSSDEKEQF421RIYRVDISDT LMYVYEMLGA ELLSNLYDKL GRLLTSSEEP YSWQHTEALL YGFQSIAETI481DVNYSDVVPG LIGLIPRISI SNVQLADTVM FTIGALSEWL ADHPVMINSV LPLVLHALGN541PELSVSSVST LKKICRECKY DLPPYAANIV AVSQDVLMKQ IHKTSQCMWL MQALGFLLSA601LQVEEILKNL HSLISPYIQQ LEKLAEEIPN PSNKLAIVHI LGLLSNLFTT LDISHHEDDH661EGPELRKLPV PQGPNPVVVV LQQVFQLIQK VLSKWLNDAQ VVEAVCAIFE KSVKTLLDDF721APMVPQLCEM LGRMYSTIPQ ASALDLTRQL VHIFAHEPAH FPPIEALFLL VTSVTLTLFQ781QGPRDHPDIV DSFMQLLAQA LKRKPDLFLC ERLDVKSVFQ CAVLALKFPE APTVKASCGF841FTELLPRCGE VESVGKVVQE DGRMLLIAVL EAIGGQASRS LMDCFADILF ALNKHCFSLL901SMWIKEALQP PGFPSARLSP EQKDTFSQQI LRERVNKRRV KEMVKEFTLL CRGLHGTDYT961ADYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
963 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.