Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IPO11 / Importin 11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101425-100
Catalog Number: LS-G101425
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IPO11 (NM_016338), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Imp11 | IPO11 | Ran binding protein 11 | RanBP11 | SLRN | Importin 11 | Importin-11 | Ran-binding protein 11
- Origin Species
- Human
- NCBI GenBank
- AK001696
- SwissProt
- Q9UI26
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 112.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDLNSASTVV LQVLTQATSQ DTAVLKPAEE QLKQWETQPG FYSVLLNIFT NHTLDINVRW61LAVLYFKHGI DRYWRRVAPH ALSEEEKTTL RAGLITNFNE PINQIATQIA VLIAKVARLD121CPRQWPELIP TLIESVKVQD DLRQHRALLT FYHVTKTLAS KRLAADRKLF YDLASGIYNF181ACSLWNHHTD TFLQEVSSGN EAAILSSLER TLLSLKVLRK LTVNGFVEPH KNMEVMGFLH241GIFERLKQFL ECSRSIGTDN VCRDRLEKTI ILFTKVLLDF LDQHPFSFTP LIQRSLEFSV301SYVFTEVGEG VTFERFIVQC MNLIKMIVKN YAYKPSKNFE DSSPETLEAH KIKMAFFTYP361TLTEICRRLV SHYFLLTEEE LTMWEEDPEG FTVEETGGDS WKYSLRPCTE VLFIDIFHEY421NQTLTPVLLE MMQTLQGPTN VEDMNALLIK DAVYNAVGLA AYELFDSVDF DQWFKNQLLP481ELQVIHNRYK PLRRRVIWLI GQWISVKFKS DLRPMLYEAI CNLLQDQDLV VRIETATTLK541LTVDDFEFRT DQFLPYLETM FTLLFQLLQQ VTECDTKMHV LHVLSCVIER VNMQIRPYVG601CLVQYLPLLW KQSEEHNMLR CAILTTLIHL VQGLGADSKN LYPFLLPVIQ LSTDVSQPPH661VYLLEDGLEL WLVTLENSPC ITPELLRIFQ NMSPLLELSS ENLRTCFKII NGYIFLSSTE721FLQTYAVGLC QSFCELLKEI TTEGQVQVLK VVENALKVNP ILGPQMFQPI LPYVFKGIIE781GERYPVVMST YLGVMGRVLL QNTSFFSSLL NEMAHKFNQE MDQLLGNMIE MWVDRMDNIT841QPERRKLSAL ALLSLLPSDN SVIQDKFCGI INISVEGLHD VMTEDPETGT YKDCMLMSHL901EEPKVTEDEE PPTEQDKRKK MLALKDPVHT VSLQQFIYEK LKAQQEMLGE QGFQSLMETV961DTEIVTQLQE FLQGFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
975 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.