Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IPCEF1 / PIP3E Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113137-100
Catalog Number: LS-G113137
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIP3E(IPCEF1) (NM_015553), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IPCEF1 | PIP3-E | RP3-402L9.2 | KIAA0403
- Origin Species
- Human
- NCBI GenBank
- AB007863
- SwissProt
- Q8WWN9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSYMAIDGS ALVPLRQKPR RKTQGFLTMS RRRISCKDLG HADCQGWLYK KKEKGSFLSN61KWKKFWVILK GSSLYWYSNQ MAEKADGFVN LPDFTVERAS ECKKKHAFKI SHPQIKTFYF121AAENVQEMNV WLNKLGSAVI HQESTTKDEE CYSESEQEDP EIAAETPPPP HASQTQSLTA181QQASSSSPSL SGTSYSFSSL ENTVKTPSSF PSSLSKERQS LPDTVNSLSA AEDEGQPITF241AVQVHSPVPS EAGIHKALEN SFVTSESGFL NSLSSDDTSS LSSNHDHLTV PDKPAGSKIM301DKEETKVSED DEMEKLYKSL EQASLSPLGD RRPSTKKELR KSFVKRCKNP SINEKLHKIR361TLNSTLKCKE HDLAMINQLL DDPKLTARKY REWKVMNTLL IQDIYQQQRA SPAPDDTDDT421PQELKKSPSS PSVENSITRTRPLEQKLISEEDLAANDILDYKDDDDKV
437 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.