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INTU Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

INTU Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all INTU antibodies →
$725.00each
Catalog Number: LS-G113058-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDZD6(INTU) (NM_015693), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Homolog of inturned | INTU | KIAA1284 | PDZK6 | PDZD6 | PDZ domain containing 6 | INT | Protein inturned
Origin Species
Human
SwissProt
Q9ULD6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASVASCDSR PSSDELPGDP SSQEEDEDYD FEDRVSDSGS YSSASSDYDD LEPEWLDSVQ
61KNGELFYLEL SEDEEESLLP ETPTVNHVRF SENEIIIEDD YKERKKYEPK LKQFTKILRR
121KRLLPKRCNK KNSNDNGPVS ILKHQSNQKT GVIVQQRYKD VNVYVNPKKL TVIKAKEQLK
181LLEVLVGIIH QTKWSWRRTG KQGDGERLVV HGLLPGGSAM KSGQVLIGDV LVAVNDVDVT
241TENIERVLSC IPGPMQVKLT FENAYDVKRE TSHPRQKKTQ SNTSDLVKLL WGEEVEGIQQ
301SGLNTPHIIM YLTLQLDSET SKEEQEILYH YPMSEASQKL KSVRGIFLTL CDMLENVTGT
361QVTSSSLLLN GKQIHVAYWK ESDKLLLIGL PAEEVPLPRL RNMIENVIQT LKFMYGSLDS
421AFCQIENVPR LDHFFNLFFQ RALQPAKLHS SASPSAQQYD ASSAVLLDNL PGVRWLTLPL
481EIKMELDMAL SDLEAADFAE LSEDYYDMRR LYTILGSSLF YKGYLICSHL PKDDLIDIAV
541YCRHYCLLPL AAKQRIGQLI IWREVFPQHH LRPLADSSTE VFPEPEGRYF LLVVGLKHYM
601LCVLLEAGGC ASKAIGSPGP DCVYVDQVKT TLHQLDGVDS RIDERLASSP VPCLSCADWF
661LTGSREKTDS LTTSPILSRL QGTSKVATSP TCRRTLFGDY SLKTRKPSPS CSSGGSDNGC
721EGGEDDGFSP HTTPDAVRKQ RESQGSDGLE ESGTLLKVTK KKSTLPNPFH LGNLKKDLPE
781KELEIYNTVK LTSGPENTLF HYVALETVQG IFITPTLEEV AQLSGSIHPQ LIKNFHQCCL
841SIRAVFQQTL VEEKKKGLNS GDHSDSAKSV SSLNPVKEHG VLFECSPGNW TDQKKAPPVM
901AYWVVGRLFL HPKPQELYVC FHDSVTEIAI EIAFKLFFGL TL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

942 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.