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INTS6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

INTS6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all INTS6 antibodies →
$504.00each
Catalog Number: LS-G114618-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX26(INTS6) (NM_012141), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DBI-1 | DBI1 | DDX26A | HDB | Integrator complex subunit 6 | Notchl2 | INT6 | INTS6 | Protein deleted in cancer 1 | RNA helicase HDB | Protein DDX26 | DDX26 | DEAD box protein | DICE1
Origin Species
Human
NCBI GenBank
NM_012141
SwissProt
Q9UL03

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPILLFLIDT SASMNQRSHL GTTYLDTAKG AVETFMKLRA RDPASRGDRY MLVTFEEPPY
61AIKAGWKENH ATFMNELKNL QAEGLTTLGQ SLRTAFDLLN LNRLVTGIDN YGQGRNPFFL
121EPAIIITITD GSKLTTTSGV QDELHLPLNS PLPGSELTKE PFRWDQRLFA LVLRLPGTMS
181VESEQLTGVP LDDSAITPMC EVTGGRSYSV CSPRMLNQCL ESLVQKVQSG VVINFEKAGP
241DPSPVEDGQP DISRPFGSQP WHSCHKLIYV RPNPKTGVPI GHWPVPESFW PDQNSPTLPP
301RTSHPVVKFS CTDCEPMVID KLPFDKYELE PSPLTQFILE RKSPQTCWQV YVSNSAKYSE
361LGHPFGYLKA STALNCVNLF VMPYNYPVLL PLLDDLFKVH KAKPTLKWRQ SFESYLKTMP
421PYYLGPLKKA VRMMGAPNLI ADSMEYGLSY SVISYLKKLS QQAKIESDRV IGSVGKKVVQ
481ETGIKVRSRS HGLSMAYRKD FQQLLQGISE DVPHRLLDLN MKEYTGFQVA LLNKDLKPQT
541FRNAYDIPRR NLLDHLTRMR SNLLKSTRRF LKGQDEDQVH SVPIAQMGNY QEYLKQVPSP
601LRELDPDQPR RLHTFGNPFK LDKKGMMIDE ADEFVAGPQN KHKRPGEPNM QGIPKRRRCM
661SPLLRGRQQN PVVNNHIGGK GPPAPTTQAQ PDLIKPLPLH KISETTNDSI IHDVVENHVA
721DQLSSDITPN AMDTEFSASS PASLLERPTN HMEALGHDHL GTNDLTVGGF LENHEEPRDK
781EQCAEENIPA SSLNKGKKLM HCRSHEEVNT ELKAQIMKEI RKPGRKYERI FTLLKHVQGS
841LQTRLIFLQN VIKEASRFKK RMLIEQLENF LDEIHRRANQ INHINSN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

887 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.