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INTS5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

INTS5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all INTS5 antibodies →
$504.00each
Catalog Number: LS-G109425-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INTS5 (NM_030628), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
INT5 | Integrator complex subunit 5 | KIAA1698 | INTS5
Origin Species
Human
SwissProt
Q6P9B9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
107.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSALCDPPGA PGPPGPAPAT HGPAPLSAQE LSQEIKAFLT GVDPILGHQL SAREHARCGL
61LLLRSLPPAR AAVLDHLRGV FDESVRAHLA ALDETPVAGP PHLRPPPPSH VPAGGPGLED
121VVQEVQQVLS EFIRANPKAW APVISAWSID LMGQLSSTYS GQHQRVPHAT GALNELLQLW
181MGCRATRTLM DIYVQCLSAL IGSCPDACVD ALLDTSVQHS PHFDWVVAHI GSSFPGTIIS
241RVLSCGLKDF CVHGGAGGGA GSSGGSSSQT PSTDPFPGSP AIPAEKRVPK IASVVGILGH
301LASRHGDSIR RELLRMFHDS LAGGSGGRSG DPSLQATVPF LLQLAVMSPA LLGTVSGELV
361DCLKPPAVLS QLQQHLQGFP REELDNMLNL AVHLVSQASG AGAYRLLQFL VDTAMPASVI
421TTQGLAVPDT VREACDRLIQ LLLLHLQKLV HHRGGSPGEG VLGPPPPPRL VPFLDALKNH
481VGELCGETLR LERKRFLWQH QLLGLLSVYT RPSCGPEALG HLLSRARSPE ELSLATQLYA
541GLVVSLSGLL PLAFRSCLAR VHAGTLQPPF TARFLRNLAL LVGWEQQGGE GPAALGAHFG
601ESASAHLSDL APLLLHPEEE VAEAAASLLA ICPFPSEALS PSQLLGLVRA GVHRFFASLR
661LHGPPGVASA CQLLTRLSQT SPAGLKAVLQ LLVEGALHRG NTELFGGQVD GDNETLSVVS
721ASLASASLLD TNRRHTAAVP GPGGIWSVFH AGVIGRGLKP PKFVQSRNQQ EVIYNTQSLL
781SLLVHCCSAP GGTECGECWG APILSPEAAK AVAVTLVESV CPDAAGAELA WPPEEHARAT
841VERDLRIGRR FREQPLLFEL LKLVAAAPPA LCYCSVLLRG LLAALLGHWE ASRHPDTTHS
901PWHLEASCTL VAVMAEGSLL PPALGNMHEV FSQLAPFEVR LLLLSVWGFL REHGPLPQKF
961IFQSERGRFI RDFSREGGGE GGPHLAVLHS VLHRNIDRLG LFSGRFQAPS PSTLLRQGT
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1019 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.