Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
INTS2 / INT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111056-100
Catalog Number: LS-G111056
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INTS2 (NM_020748), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Integrator complex subunit 2 | INT2 | INTS2 | KIAA1287
- Origin Species
- Human
- NCBI GenBank
- AL136800
- SwissProt
- Q9H0H0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 134.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ61SQSWAQDKKL ILRLLSGVEA VNSIVALLSV DFHALEQDAS KEQQLRHKLG GGSGESILVS121QLQHGLTLEF EHSDSPRRLR LVLSELLAIM NKVSESNGEF FFKSSELFES PVYLEEAADV181LCILQAELPS LLPIVDVAEA LLHVRNGAWF LCLLVANVPD SFNEVCRGLI KNGERQDEES241LGGRRRTDAL RFLCKMNPSQ ALKVRGMVVE ECHLPGLGVA LTLDHTKNEA CEDGVSDLVC301FVSGLLLGTN AKVRTWFGTF IRNGQQRKRE TSSSVLWQMR RQLLLELMGI LPTVRSTRIV361EEADVDMEPN VSVYSGLKEE HVVKASALLR LYCALMGIAG LKPTEEEAEQ LLQLMTSRPP421ATPAGVRFVS LSFCMLLAFS TLVSTPEQEQ LMVVWLSWMI KEEAYFESTS GVSASFGEML481LLVAMYFHSN QLSAIIDLVC STLGMKIVIK PSSLSRMKTI FTQEIFTEQV VTAHAVRVPV541TSNLSANITG FLPIHCIYQL LRSRSFTKHK VSIKDWIYRQ LCETSTPLHP QLLPLIDVYI601NSILTPASKS NPEATNQPVT EQEILNIFQG VIGGDNIRLN QRFSITAQLL VLYYILSYEE661ALLANTKTLA AMQRKPKSYS SSLMDQIPIK FLIRQAQGLQ QELGGLHSAL LRLLATNYPH721LCIVDDWICE EEITGTDALL RRMLLTNNAK NHSPKQLQEA FSAVPVNHTQ VMQIIEHLTL781LSASELIPYA EVLTSNMSQL LNSGVPRRIL QTVNKLWMVL NTVMPRRLWV MTVNALQPSI841KFVRQQKYTQ NDLMIDPLIV LRCDQRVHRC PPLMDITLHM LNGYLLASKA YLSAHLKETE901QDRPSQNNTI GLVGQTDAPE VTREELKNAL LAAQDSAAVQ ILLEICLPTE EEKANGVNPD961SLLRNVQSVI TTSAPNKGME EGEDNLLCNL REVQCLICCL LHQMYIADPN IAKLVHFQGY1021PCELLPLTVA GIPSMHICLD FIPELIAQPE LEKQIFAIQL LSHLCIQYAL PKSLSVARLA1081VNVMGTLLTV LTQAKRYAFF MPTLPSLVSF CRAFPPLYED IMSLLIQIGQ VCASDVATQT1141RDIDPIITRL QQIKEKPSGW SQICKDSSYK NGSRDTGSMD PDVQLCHCIE RTVIEIINMS1201VSGITRTRPLEQKLISEEDLAANDILDYKDDDDKV
1204 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.