Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
INT7 / INTS7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113221-100
Catalog Number: LS-G113221
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INTS7 (NM_015434), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Integrator complex subunit 7 | C1orf73 | DKFZP434B168 | INT7 | INTS7
- Origin Species
- Human
- NCBI GenBank
- NM_015434
- SwissProt
- Q9NVH2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 106.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASNSTKSFL ADAGYGEQEL DANSALMELD KGLRSGKLGE QCEAVVRFPR LFQKYPFPIL61INSAFLKLAD VFRVGNNFLR LCVLKVTQQS EKHLEKILNV DEFVKRIFSV IHSNDPVARA121ITLRMLGSLA SIIPERKNAH HSIRQSLDSH DNVEVEAAVF AAANFSAQSK DFAVGICNKI181SEMIQGLATP VDLKLKLIPI LQHMHHDAIL ASSARQLLQQ LVTSYPSTKM VIVSLHTFTL241LAASSLVDTP KQIQLLLQYL KNDPRKAVKR LAIQDLKLLA NKTPHTWSRE NIQALCECAL301QTPYDSLKLG MLSVLSTLSG TIAIKHYFSI VPGNVSSSPR SSDLVKLAQE CCYHNNRGIA361AHGVRVLTNI TVSCQEKDLL ALEQDAVFGL ESLLVLCSQD DSPGAQATLK IALNCMVKLA421KGRPRLSQSV VETLLTQLHS AQDAARILMC HCLAAIAMQL PVLGDGMLGD LMELYKVIGR481SATDKQQELL VSLATVIFVA SQKALSVESK AVIKQQLESV SNGWTVYRIA RQASRMGNHD541MAKELYQSLL TQVASEHFYF WLNSLKEFSH AEQCLTGLQE ENYSSALSCI AESLKFYHKG601IASLTAASTP LNPLSFQCEF VKLRIDLLQA FSQLICTCNS LKTSPPPAIA TTIAMTLGND661LQRCGRISNQ MKQSMEEFRS LASRYGDLYQ ASFDADSATL RNVELQQQSC LLISHAIEAL721ILDPESASFQ EYGSTGTAHA DSEYERRMMS VYNHVLEEVE SLNRKYTPVS YMHTACLCNA781IIALLKVPLS FQRYFFQKLQ STSIKLALSP SPRNPAEPIA VQNNQQLALK VEGVVQHGSK841PGLFRKIQSV CLNVSSTLQS KSGQDYKIPI DNMTNEMEQR VEPHNDYFST QFLLNFAILG901THNITVESSV KDANGIVWKT GPRTTIFVKS LEDPYSQQIR LQQQQAQQPL QQQQQRNAYT961RFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
962 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.