INT1 / INTS1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119328-100
Catalog Number: LS-G119328
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INTS1 (NM_001080453), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- INTS1 | INT1 | Integrator complex subunit 1 | KIAA1440 | NET28
- Origin Species
- Human
- NCBI GenBank
- AB037861
- SwissProt
- Q8N201
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 244.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNRAKPTTVR RPSAAAKPSG HPPPGDFIAL GSKGQANESK TASTLLKPAP SGLPSERKRD61AAAALSSASA LTGLTKRPKL SSTPPLSALG RLAEAAVAEK RAISPSIKEP SVVPIEVLPT121VLLDEIEAAE LEGNDDRIEG VLCGAVKQLK VTRAKPDSTL YLSLMYLAKI KPNIFATEGV181IEALCSLLRR DASINFKAKG NSLVSVLACN LLMAAYEEDE NWPEIFVKVY IEDSLGERIW241VDSPHCKTFV DNIQTAFNTR MPPRSVLLQG EAGRVAGDLG AGSSPHPSLT EEEDSQTELL301IAEEKLSPEQ EGQLMPRYEE LAESVEEYVL DMLRDQLNRR QPIDNVSRNL LRLLTSTCGY361KEVRLLAVQK LEMWLQNPKL TRPAQDLLMS VCMNCNTHGS EDMDVISHLI KIRLKPKVLL421NHFMLCIREL LSAHKDNLGT TIKLVIFNEL SSARNPNNMQ VLYTALQHSS ELAPKFLAMV481FQDLLTNKDD YLRASRALLR EIIKQTKHEI NFQAFCLGLM QERKEPQYLE MEFKERFVVH541ITDVLAVSMM LGITAQVKEA GIAWDKGEKR NLEVLRSFQN QIAAIQRDAV WWLHTVVPSI601SKLAPKDYVH CLHKVLFTEQ PETYYKWDNW PPESDRNFFL RLCSEVPILE DTLMRILVIG661LSRELPLGPA DAMELADHLV KRAAAVQADD VEVLKVGRTQ LIDAVLNLCT YHHPENIQLP721PGYQPPNLAI STLYWKAWPL LLVVAAFNPE NIGLAAWEEY PTLKMLMEMV MTNNYSYPPC781TLTDEETRTE MLNRELQTAQ REKQEILAFE GHLAAASTKQ TITESSSLLL SQLTSLDPQG841PPRRPPPHIL DQVKSLNQSL RLGHLLCRSR NPDFLLHIIQ RQASSQSMPW LADLVQSSEG901SLDVLPVQCL CEFLLHDAVD DAASGEEDDE GESKEQKAKK RQRQQKQRQL LGRLQDLLLG961PKADEQTTCE VLDYFLRRLG SSQVASRVLA MKGLSLVLSE GSLRDGEEKE PPMEEDVGDT1021DVLQGYQWLL RDLPRLPLFD SVRSTTALAL QQAIHMETDP QTISAYLIYL SQHTPVEEQA1081QHSDLALDVA RLVVERSTIM SHLFSKLSPS AASDAVLSAL LSIFSRYVRR MRQSKEGEEV1141YSWSESQDQV FLRWSSGETA TMHILVVHAM VILLTLGPPR ADDSEFQALL DIWFPEEKPL1201PTAFLVDTSE EALLLPDWLK LRMIRSEVLR LVDAALQDLE PQQLLLFVQS FGIPVSSMSK1261LLQFLDQAVA HDPQTLEQNI MDKNYMAHLV EVQHERGASG GQTFHSLLTA SLPPRRDSTE1321APKPKSSPEQ PIGQGRIRVG TQLRVLGPED DLAGMFLQIF PLSPDPRWQS SSPRPVALAL1381QQALGQELAR VVQGSPEVPG ITVRVLQALA TLLSSPHGGA LVMSMHRSHF LACPLLRQLC1441QYQRCVPQDT GFSSLFLKVL LQMLQWLDSP GVEGGPLRAQ LRMLASQASA GRRLSDVRGG1501LLRLAEALAF RQDLEVVSST VRAVIATLRS GEQCSVEPDL ISKVLQGLIE VRSPHLEELL1561TAFFSATADA ASPFPACKPV VVVSSLLLQE EEPLAGGKPG ADGGSLEAVR LGPSSGLLVD1621WLEMLDPEVV SSCPDLQLRL LFSRRKGKGQ AQVPSFRPYL LTLFTHQSSW PTLHQCIRVL1681LGKSREQRFD PSASLDFLWA CIHVPRIWQG RDQRTPQKRR EELVLRVQGP ELISLVELIL1741AEAETRSQDG DTAACSLIQA RLPLLLSCCC GDDESVRKVT EHLSGCIQQW GDSVLGRRCR1801DLLLQLYLQR PELRVPVPEV LLHSEGAASS SVCKLDGLIH RFITLLADTS DSRALENRGA1861DASMACRKLA VAHPLLLLRH LPMIAALLHG RTHLNFQEFR QQNHLSCFLH VLGLLELLQP1921HVFRSEHQGA LWDCLLSFIR LLLNYRKSSR HLAAFINKFV QFIHKYITYN APAAISFLQK1981HADPLHDLSF DNSDLVMLKS LLAGLSLPSR DDRTDRGLDE EGEEESSAGS LPLVSVSLFT2041PLTAAEMAPY MKRLSRGQTV EDLLEVLSDI DEMSRRRPEI LSFFSTNLQR LMSSAEECCR2101NLAFSLALRS MQNSPSIAAA FLPTFMYCLG SQDFEVVQTA LRNLPEYALL CQEHAAVLLH2161RAFLVGMYGQ MDPSAQISEA LRILHMEAVMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
2190 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.