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INSM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

INSM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all INSM1 antibodies →
$725.00each
Catalog Number: LS-G118134-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INSM1 (NM_002196), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
INSM1 | Insulinoma-associated 1 | IA-1 | Zinc finger protein IA-1 | IA1
Origin Species
Human
NCBI GenBank
NM_002196
SwissProt
Q01101

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MPRGFLVKRS KKSTPVSYRV RGGEDGDRAL LLSPSCGGAR AEPPAPSPVP GPLPPPPPAE
61RAHAALAAAL ACAPGPQPPP QGPRAAHFGN PEAAHPAPLY SPTRPVSREH EKHKYFERSF
121NLGSPVSAES FPTPAALLGG GGGGGASGAG GGGTCGGDPL LFAPAELKMG TAFSAGAEAA
181RGPGPGPPLP PAAALRPPGK RPPPPTAAEP PAKAVKAPGA KKPKAIRKLH FEDEVTTSPV
241LGLKIKEGPV EAPRGRAGGA ARPLGEFICQ LCKEEYADPF ALAQHKCSRI VRVEYRCPEC
301AKVFSCPANL ASHRRWHKPR PAPAAARAPE PEAAARAEAR EAPGGGSDRD TPSPGGVSES
361GSEDGLYECH HCAKKFRRQA YLRKHLLAHH QALQAKGAPL APPAEDLLAL YPGPDEKAPQ
421EAAGDGEGAG VLGLSASAEC HLCPVCGESF ASKGAQERHL RLLHAAQVFP CKYCPATFYS
481SPGLTRHINK CHPSENRQVI LLQVPVRPAC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

510 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.