Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
INPP5J / PIB5PA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122643-100
Catalog Number: LS-G122643
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIB5PA(INPP5J) (NM_001002837), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PIB5PA | INPP5 | PIPP | INPP5J
- Origin Species
- Human
- NCBI GenBank
- U45975
- SwissProt
- Q15735
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGQSSRGSR RPGTRAGLGS LPMPQGVAQT GAPSKVDSSF QLPAKKNAAL GPSEPRITVV61TWNVGTAMPP DDVTSLLHLG GGDDSDGADM IAIGLQEVNS MLNKRLKDAL FTDQWSELFM121DALGPFNFVL VSSVRMQGVI LLLFAKYYHL PFLRDVQTDC TRTGLGGYWG NKGGVSVRLA181AFGHMLCFLN CHLPAHMDKA EQRKDNFQTI LSLQQFQGPG AQGILDHDLV FWFGDLNFRI241ESYDLHFVKF AIDSDQLHQL WEKDQLNMAK NTWPILKGFQ EGPLNFAPTF KFDVGTNKYD301TSAKKRKPAW TDRILWKVKA PGGGPSPSGR KSHRLQVTQH SYRSHMEYTV SDHKPVAAQF361LLQFAFRDDM PLVRLEVADE WVRPEQAVVR YRMETVFARS SWDWIGLYRV GFRHCKDYVA421YVRAKHEDVD GNTYQVTFSE ESLPKGHGDF ILGYYSHNHS ILIGITEPFQ ISLPSSELAS481SSTDSSGTSS EGEDDSTLEL LAPKSRSPSP GKSKRHRSRS PGLARFPGLA LRPSSRERRG541ASRSPSPQSR RLSRVAPDRS SNGSSRGSSE EGPSGLPGPW AFPPAVPRSL GLLPALRLET601VDPGGGGSWG PDREALAPNS LSPSPQGHRG LEEGGLGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
638 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.