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INPP5F Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

INPP5F Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all INPP5F antibodies →
$504.00each
Catalog Number: LS-G113570-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INPP5F (NM_014937), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSAC2 | INPP5F | KIAA0966 | MSTP007 | MSTPO47 | SAC2
Origin Species
Human
NCBI GenBank
NM_014937
SwissProt
Q9Y2H2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
128.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELFQAKDHY ILQQGERALW CSRRDGGLQL RPATDLLLAW NPICLGLVEG VIGKIQLHSD
61LPWWLILIRQ KALVGKLPGD HEVCKVTKIA VLSLSEMEPQ DLELELCKKH HFGINKPEKI
121IPSPDDSKFL LKTFTHIKSN VSAPNKKKVK ESKEKEKLER RLLEELLKMF MDSESFYYSL
181TYDLTNSVQR QSTGERDGRP LWQKVDDRFF WNKYMIQDLT EIGTPDVDFW IIPMIQGFVQ
241IEELVVNYTE SSDDEKSSPE TPPQESTCVD DIHPRFLVAL ISRRSRHRAG MRYKRRGVDK
301NGNVANYVET EQLIHVHNHT LSFVQTRGSV PVFWSQVGYR YNPRPRLDRS EKETVAYFCA
361HFEEQLNIYK KQVIINLVDQ AGREKVIGDA YLKQVLLFNN SHLTYVSFDF HEHCRGMKFE
421NVQTLTDAIY DIILDMKWCW VDEAGVICKQ EGIFRVNCMD CLDRTNVVQA AIARVVMEQQ
481LKKLGVMPPE QPLPVKCNRI YQIMWANNGD SISRQYAGTA ALKGDFTRTG ERKLAGVMKD
541GVNSANRYYL NRFKDAYRQA VIDLMQGIPV TEDLYSIFTK EKEHEALHKE NQRSHQELIS
601QLLQSYMKLL LPDDEKFHGG WALIDCDPSL IDATHRDVDV LLLLSNSAYY VAYYDDEVDK
661VNQYQRLSLE NLEKIEIGPE PTLFGKPKFS CMRLHYRYKE ASGYFHTLRA VMRNPEEDGK
721DTLQCIAEML QITKQAMGSD LPIIEKKLER KSSKPHEDII GIRSQNQGSL AQGKNFLMSK
781FSSLNQKVKQ TKSNVNIGNL RKLGNFTKPE MKVNFLKPNL KVNLWKSDSS LETMENTGVM
841DKVQAESDGD MSSDNDSYHS DEFLTNSKSD EDRQLANSLE SVGPIDYVLP SCGIIASAPR
901LGSRSQSLSS TDSSVHAPSE ITVAHGSGLG KGQESPLKKS PSAGDVHILT GFAKPMDIYC
961HRFVQDAQNK VTHLSETRSV SQQASQERNQ MTNQVSDETQ SESTEQTPSR PSQLDVSLSA
1021TGPQFLSVEP AHSVASQKTP TSASSMLELE TGLHVTPSPS ESSSSRAVSP FAKIRSSMVQ
1081VASITQAGLT HGINFAVSKV QKSPPEPEII NQVQQNELKK MFIQCQTRII QI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1132 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.