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INPP4B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

INPP4B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all INPP4B antibodies →
$725.00each
Catalog Number: LS-G118968-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INPP4B (NM_001101669), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
INPP4B
Origin Species
Human
NCBI GenBank
NM_003866
SwissProt
O15327

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
104.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEIKEEGASE EGQHFLPTAQ ANDPGDCQFT SIQKTPNEPQ LEFILACKDL VAPVRDRKLN
61TLVQISVIHP VEQSLTRYSS TEIVEGTRDP LFLTGVTFPS EYPIYEETKI KLTVYDVKDK
121SHDTVRTSVL PEHKDPPPEV GRSFLGYASF KVGELLKSKE QLLVLSLRTS DGGKVVGTIE
181VSVVKMGEIE DGEADHITTD VQGQKCALVC ECTAPESVSG KDNLPFLNSV LKNPVCKLYR
241FPTSDNKWMR IREQMSESIL SFHIPKELIS LHIKEDLCRN QEIKELGELS PHWDNLRKNV
301LTHCDQMVNM YQDILTELSK ETGSSFKSSS SKGEKTLEFV PINLHLQRMQ VHSPHLKDAL
361YDVITVGAPA AHFQGFKNGG LRKLLHRFET ERRNTGYQFI YYSPENTAKA KEVLSNINQL
421QPLIATHADL LLNSASQHSP DSLKNSLKML SEKTELFVHA FKDQLVRSAL LALYTARPGG
481ILKKPPSPKS STEESSPQDQ PPVMRGQDSI PHHSDYDEEE WDRVWANVGK SLNCIIAMVD
541KLIERDGGSE GSGGNNDGEK EPSLTDAIPS HPREDWYEQL YPLILTLKDC MGEVVNRAKQ
601SLTFVLLQEL AYSLPQCLML TLRRDIVFSQ ALAGLVCGFI IKLQTSLYDP GFLQQLHTVG
661LIVQYEGLLS TYSDEIGMLE DMAVGISDLK KVAFKIIEAK SNDVLPVITG RREHYVVEVK
721LPARMFESLP LQIKEGQLLH VYPVLFNVGI NEQQTLAERF GDVSLQESIN QENFELLQEY
781YKIFMEKMPP DYISHFQEQN DLKALLENLL QNIQSKKRKN VEIMWLAATI CRKLNGIRFT
841CCKSAKDRTS MSVTLEQCSI LRDEHQLHKD FFIRALDCMR REGCRIENVL KNIKCRKYAF
901NMLQLMAFPK YYRPPEGTYG KADT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

924 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.