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INPP4A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

INPP4A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all INPP4A antibodies →
$725.00each
Catalog Number: LS-G118510-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INPP4A (NM_001566), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
INPP4A | TVAS1 | INPP4
Origin Species
Human
NCBI GenBank
NM_001566
SwissProt
Q96PE3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
107.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTAREHSPRH GARARAMQRA STIDVAADML GLSLAGNIQD PDEPILEFSL ACSELHTPSL
61DRKPNSFVAV SVTTPPQAFW TKHAQTEIIE GTNNPIFLSS IAFFQDSLIN QMTQVKLSVY
121DVKDRSQGTM YLLGSGTFIV KDLLQDRHHR LHLTLRSAES DRVGNITVIG WQMEEKSDQR
181PPVTRSVDTV NGRMVLPVDE SLTEALGIRS KYASLRKDTL LKSVFGGAIC RMYRFPTTDG
241NHLRILEQMA ESVLSLHVPR QFVKLLLEED AARVCELEEL GELSPCWESL RRQIVTQYQT
301IILTYQENLT DLHQYRGPSF KASSLKADKK LEFVPTNLHI QRMRVQDDGG SDQNYDIVTI
361GAPAAHCQGF KSGGLRKKLH KFEETKKHFE ECCTSSGCQS IIYIPQDVVR AKEIIAQINT
421LKTQVSYYAE RLSRAAKDRS ATGLERTLAI LADKTRQLVT VCDCKLLANS IHGLNAARPD
481YIASKASPTS TEEEQVMLRN DQDTLMARWT GRNSRSSLQV DWHEEEWEKV WLNVDKSLEC
541IIQRVDKLLQ KERLHGEGCE DVFPCAGSCT SKKDCSPPPE ESSPGEWSEA LYPLLTTLTD
601CVAMMSDKAK KAMVFLLMQD SAPTIATYLS LQYRRDVVFC QTLTALICGF IIKLRNCLHD
661DGFLRQLYTI GLLAQFESLL STYGEELAML EDMSLGIMDL RNVTFKVTQA TSSASADMLP
721VITGNRDGFN VRVPLPGPLF DALPREIQSG MLLRVQPVLF NVGINEQQTL AERFGDTSLQ
781EVINVESLVR LNSYFEQFKE VLPEDCLPRS RSQTCLPELL RFLGQNVHAR KNKNVDILWQ
841AAEICRRLNG VRFTSCKSAK DRTAMSVTLE QCLILQHEHG MAPQVFTQAL ECMRSIGTRE
901VVTQKNLSGL VPIRDLRLDP SLLCSIPLLA LSPNLLIVWL FLSIAYLVTK LRCK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

954 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.