Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
INO80B / PAPA-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109272-100
Catalog Number: LS-G109272
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INO80B (NM_031288), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HIes2 | IES2 | INO80 complex subunit B | HMGA1L4 | PAPA1 | PAP-1-associated protein 1 | PAP-1BP | Zinc finger | HIT type 4 | PAPA-1 | HMGIYL4 | IES2 homolog | INO80B | PAP-1 binding protein | ZNHIT4
- Origin Species
- Human
- NCBI GenBank
- NM_031288
- SwissProt
- Q9C086
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAPEPGEAL ELSLAGAHGH GVHKKKHKKH KKKHKKKHHQ EEDAGPTQPS PAKPQLKLKI61KLGGQVLGTK SVPTFTVIPE GPRSPSPLMV VDNEEEPMEG VPLEQYRAWL DEDSNLSPSP121LRDLSGGLGG QEEEEEQRWL DALEKGELDD NGDLKKEINE RLLTARQRAL LQKARSQPSP181MLPLPVAEGC PPPALTEEML LKREERARKR RLQAARRAEE HKNQTIERLT KTAATSGRGG241RGGARGERRG GRAAAPAPMV RYCSGAQGST LSFPPGVPAP TAVSQRPSPS GPPPRCSVPG301CPHPRRYACS RTGQALCSLQ CYRINLQMRL GGPEGPGSPL LATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
343 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.