Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
INHBE / Activin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109168-100
Catalog Number: LS-G109168
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Inhibin beta E chain(INHBE) (NM_031479), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Activin beta E | Activin beta-E chain | Activin | INHBE | Inhibin beta E chain | Inhibin | beta E
- Origin Species
- Human
- NCBI GenBank
- NM_031479
- SwissProt
- P58166
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRLPDVQLWL VLLWALVRAQ GTGSVCPSCG GSKLAPQAER ALVLELAKQQ ILDGLHLTSR61PRITHPPPQA ALTRALRRLQ PGSVAPGNGE EVISFATVTD STSAYSSLLT FHLSTPRSHH121LYHARLWLHV LPTLPGTLCL RIFRWGPRRR RQGSRTLLAE HHITNLGWHT LTLPSSGLRG181EKSGVLKLQL DCRPLEGNST VTGQPRRLLD TAGHQQPFLE LKIRANEPGA GRARRRTPTC241EPATPLCCRR DHYVDFQELG WRDWILQPEG YQLNYCSGQC PPHLAGSPGI AASFHSAVFS301LLKANNPWPA STSCCVPTAR RPLSLLYLDH NGNVVKTDVP DMVVEACGCSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
350 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.