Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
INHBB / Inhibin Beta B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118131-100
Catalog Number: LS-G118131
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Inhibin beta B(INHBB) (NM_002193), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Activin beta-B chain | Activin AB beta polypeptide | INHBB | Inhibin | beta B | Inhibin Beta B | Inhibin beta B chain | beta-2
- Origin Species
- Human
- NCBI GenBank
- NM_002193
- SwissProt
- P09529
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.18 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDGLPGRALG AACLLLLAAG WLGPEAWGSP TPPPTPAAQP PPPPPGSPGG SQDTCTSCGG61FRRPEELGRV DGDFLEAVKR HILSRLQMRG RPNITHAVPK AAMVTALRKL HAGKVREDGR121VEIPHLDGHA SPGADGQERV SEIISFAETD GLASSRVRLY FFISNEGNQN LFVVQASLWL181YLKLLPYVLE KGSRRKVRVK VYFQEQGHGD RWNMVEKRVD LKRSGWHTFP LTEAIQALFE241RGERRLNLDV QCDSCQELAV VPVFVDPGEE SHRPFVVVQA RLGDSRHRIR KRGLECDGRT301NLCCRQQFFI DFRLIGWNDW IIAPTSYYGN YCEGSCPAYL AGVPGSASSF HTAVVNQYRM361RGLNPGTVNS CCIPTKLSTM SMLYFDDEYN IVKRDVPNMI VEECGCATRTRPLEQKLISEEDLAANDILDYKDDDDKV
407 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.