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IMPG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IMPG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IMPG1 antibodies →
$725.00each
Catalog Number: LS-G118507-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IMPG1 (NM_001563), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IMPG1 | IPM-150 | SPACR | gp147 | IPM150
Origin Species
Human
NCBI GenBank
NM_001563
SwissProt
Q17R60

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYLETRRAIF VFWIFLQVQG TKDISINIYH SETKDIDNPP RNETTESTEK MYKMSTMRRI
61FDLAKHRTKR SAFFPTGVKV CPQESMKQIL DSLQAYYRLR VCQEAVWEAY RIFLDRIPDT
121GEYQDWVSIC QQETFCLFDI GKNFSNSQEH LDLLQQRIKQ RSFPDRKDEI SAEKTLGEPG
181ETIVISTDVA NVSLGPFPLT PDDTLLNEIL DNTLNDTKMP TTERETEFAV LEEQRVELSV
241SLVNQKFKAE LADSQSPYYQ ELAGKSQLQM QKIFKKLPGF KKIHVLGFRP KKEKDGSSST
301EMQLTAIFKR HSAEAKSPAS DLLSFDSNKI ESEEVYHGTM EEDKQPEIYL TATDLKRLIS
361KALEEEQSLD VGTIQFTDEI AGSLPAFGPD TQSELPTSFA VITEDATLSP ELPPVEPQLE
421TVDGAEHGLP DTSWSPPAMA STSLSEAPPF FMASSIFSLT DQGTTDTMAT DQTMLVPGLT
481IPTSDYSAIS QLALGISHPP ASSDDSRSSA GGEDMVRDLD EMDLSDTPAP SEVPELSEYV
541SVPDHFLEDT TPVSALQYIT TSSMTIAPKG RELVVFFSLR VANMAFSNDL FNKSSLEYRA
601LEQQFTQLLV PYLRSNLTGF KQLEILNFRN GSVIVNSKMK FAKSVPYNLT KAVHGVLEDF
661RSAAAQQLHL EIDSYSLNIE PADQADPCKF LACGEFAQCV KNEWTEEAEC RCKPGYDSQG
721SLDGLEPGLC GPGTKECEVL QGKGAPCRLP DHSENQAYKT SVKKFQNQQN NKVISKRNSE
781LLTVEYEEFN HQDWEGN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

797 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.