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IMPDH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IMPDH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IMPDH1 antibodies →
$504.00each
Catalog Number: LS-G126022-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IMPDH1 (NM_001142575), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IMP dehydrogenase 1 | IMPD 1 | IMPDH 1 | IMPDH1 | IMPD1 | LCA11 | IMPD | IMPDH-I | SWSS2608 | RP10
Origin Species
Human
NCBI GenBank
NM_000883
SwissProt
P20839

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MADYLISGGT GYVPEDGLTA QQLFASADGL TYNDFLILPG FIDFIADEVD LTSALTRKIT
61LKTPLISSPM DTVTEADMAI AMAKFEQGFI TDPVVLSPSH TVGDVLEAKM RHGFSGIPIT
121ETGTMGSKLV GIVTSRDIDF LAEKDHTTLL SEVMTPRIEL VVAPAGVTLK EANEILQRSK
181KGKLPIVNDC DELVAIIART DLKKNRDYPL ASKDSQKQLL CGAAVGTRED DKYRLDLLTQ
241AGVDVIVLDS SQGNSVYQIA MVHYIKQKYP HLQVIGGNVV TAAQAKNLID AGVDGLRVGM
301GCGSICITQE VMACGRPQGT AVYKVAEYAR RFGVPIIADG GIQTVGHVVK ALALGASTVM
361MGSLLAATTE APGEYFFSDG VRLKKYRGMG SLDAMEKSSS SQKRYFSEGD KVKIAQGVSG
421SIQDKGSIQK FVPYLIAGIQ HGCQDIGARS LSVLRSMMYS GELKFEKRTM SAQIEGGVHG
481LHSYEKRLY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

489 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.